CD164 Polyclonal antibody

CD164 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 12083-2-AP

Synonyms

CD164, CD164 molecule, sialomucin, endolyn, MUC 24, Sialomucin core protein 24

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Validation Data Gallery

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  WB analysis using 12083-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12083-2-AP (CD164 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 12083-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12083-2-AP (CD164 antibody) at dilution of 1:50 (under 10x lens).

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  FC experiment of K-562 using 12083-2-AP

  1X10^6 K-562 cells were intracellularly stained with 0.5 ug Anti-Human CD164 (12083-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.5 ug Control Antibody. Cells were fixed with 90% MeOH .

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Tested Applications

• Positive WB detected in: BxPC-3 cells, U-87 MG cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12083-2-AP targets CD164 in WB, IHC, FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD164 fusion protein Ag2715
• Full Name: CD164 molecule, sialomucin
• Calculated Molecular Weight: 197 aa, 21 kDa
• Observed Molecular Weight: 82 kDa
• GenBank Accession Number: BC011522
• Gene Symbol: CD164
• Gene ID (NCBI): 8763
• RRID: AB_2072583
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sialomucins are a heterogeneous group of secreted or membrane-associated mucins that appear to play 2 key but opposing roles in vivo: first as cytoprotective or antiadhesive agents, and second as adhesion receptors. CD164 is a type I integral transmembrane sialomucin that functions as an adhesion receptor (PMID: 9680353)(PMID: 17077324). Sialomucin CD164 (MUC-24), also referred to multi-glycosylated core protein 24 (MGC24), is known to function as a receptor that regulates stem cell localization to the bone marrow. CD164 may play a key role in hematopoiesis by facilitating the adhesion of CD34+ cells to the stroma and by negatively regulating CD34+CD38(lo/-) cell proliferation. Important role of CD164 in in prostate cancer metastasis, promoting myogenesis and regulating myoblast migration so far have been revealed (PMID: 9763543)(PMID: 16859559)(PMID: 17077324).