Anti-Human CD16b Rabbit Recombinant Antibody, PBS Only

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  FC experiment of human peripheral blood leukocytes using 98192-1-RR (same clone as 98192-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD16b Rabbit Recombinant Antibody (98192-1-RR, Clone:241713E3) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. This data was developed using the same antibody clone with 98192-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human peripheral blood leukocytes using 98192-1-RR (same clone as 98192-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD16b Rabbit Recombinant Antibody (98192-1-RR, Clone:241713E3) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. This data was developed using the same antibody clone with 98192-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98192-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98192-1-RR against Human CD16b were performed. The affinity constant is 2.36 nM.

CD16b Uni-rAb^® Recombinant Antibody for FC

Cat No. 98192-1-PBS

Clone No.241713E3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD16, FCGR3B, 241713E3, Fc gamma RIII, Fc gamma RIII beta

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Product Information

98192-1-PBS targets CD16b in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2487

  Product name: recombinant human CD16b protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 10007018 of

  Sequence:

• Full Name: Fc fragment of IgG, low affinity IIIb, receptor (CD16b)
• Gene Symbol: FCGR3B
• Gene ID (NCBI): 2215
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD16 is a low affinity Fc receptor. It has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and FCGR3B. In humans, FcγRIIIa/CD16a is a type I transmembrane receptor expressed on macrophages and NK cells. FcγRIIIb/CD16b is a GPI-linked Fc-receptor highly expressed on neutrophils. The two proteins differ by only four amino acid residues in the mature proteins (PMID: 30361439).