FITC Plus Anti-Human CD177 Rabbit Recombinant Antibody

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  FC experiment of human peripheral blood leukocytes using FITC-98086

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul FITC Plus Anti-Human CD177 Rabbit RecAb (FITC-98086, Clone: 241339F4) or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using FITC-98086

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul FITC Plus Anti-Human CD177 Rabbit RecAb (FITC-98086, Clone: 241339F4)(red) or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

CD177 Uni-rAb^® Recombinant Antibody for FC

Cat No. FITC-98086

Clone No.241339F4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

241339F4, HNA 2a, HNA2A, HNA-2a, Human neutrophil alloantigen 2a

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

FITC-98086 targets CD177 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: CD177 molecule
• Calculated Molecular Weight: 437 aa, 46 kDa
• GenBank Accession Number: BC029167
• Gene Symbol: CD177
• Gene ID (NCBI): 57126
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8N6Q3
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD177, also known as NB1, HNA-2a or PRV-1, is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein that belongs to the uPAR/CD59/Ly6 snake toxin superfamily (PMID: 17898576). It is expressed exclusively by neutrophils, neutrophilic metamyelocytes, and myelocytes (PMID: 17580308). CD177 interacts with β2 integrin CD11b/CD18 and CD11a/CD18, PECAM-1, and participates in neutrophil activation and transmigration (PMID: 17580308; 21193407). CD177 has been identified as a useful biomarker for myeloproliferative diseases (PMID: 17898576).