FITC Anti-Human CD18 (TS1/18)

CD18 Monoclonal Antibody for FC

Host / Isotype

Mouse / Mouse IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

FITC Fluorescent Dye

CloneNo.

TS1/18

Cat no : FITC-65190

Synonyms

CD18, Integrin beta 2, ITGB2, LAD, LCAMB, LFA 1, MAC 1, MF17, MFI7



Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

FITC-65190 targets CD18 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / Mouse IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human Beta 2 Integrin
Full Name integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
Calculated molecular weight 85 kDa
GenBank accession numberBC005861
Gene symbol ITGB2
Gene ID (NCBI) 3689
Conjugate FITC Fluorescent Dye
Excitation/Emission maxima wavelengths494 nm / 520 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during immune response and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).

Protocols

Product Specific Protocols
FC protocol for FITC CD18 antibody FITC-65190Download protocol
Standard Protocols
Click here to view our Standard Protocols