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CD1d Monoclonal antibody

CD1d Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C12F1

Cat no : 66257-1-Ig

Synonyms

CD1A, CD1D, CD1d molecule, R3, R3G1



Tested Applications

Positive WB detected inhuman testis tissue, Jurkat
Positive IHC detected inhuman skin tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

66257-1-Ig targets CD1d in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD1d fusion protein Ag11108
Full Name CD1d molecule
Calculated molecular weight 335 aa, 38 kDa
Observed molecular weight 40 kDa, 50-55 kDa
GenBank accession numberBC027926
Gene symbol CD1D
Gene ID (NCBI) 912
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD1d is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. CD1d is an antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on NKT cells. When activated, NKT cells rapidly produce Th1 and Th2 cytokines. The molecular weight of unglycosylated CD1d is 38 kDa, while glycosylated form of CD1d is 48-55 kDa.

Protocols

Product Specific Protocols
WB protocol for CD1d antibody 66257-1-IgDownload protocol
IHC protocol for CD1d antibody 66257-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols