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CD1d Monoclonal antibody

CD1d Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1









Cat no : 66257-1-Ig


CD1A, CD1D, CD1d molecule, R3, R3G1

Tested Applications

Positive WB detected inhuman testis tissue, Jurkat
Positive IHC detected inhuman skin tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66257-1-Ig targets CD1d in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD1d fusion protein Ag11108
Full Name CD1d molecule
Calculated Molecular Weight 335 aa, 38 kDa
Observed Molecular Weight 40 kDa, 50-55 kDa
GenBank Accession NumberBC027926
Gene Symbol CD1D
Gene ID (NCBI) 912
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD1d is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. CD1d is an antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on NKT cells. When activated, NKT cells rapidly produce Th1 and Th2 cytokines. The molecular weight of unglycosylated CD1d is 38 kDa, while glycosylated form of CD1d is 48-55 kDa.


Product Specific Protocols
WB protocol for CD1d antibody 66257-1-IgDownload protocol
IHC protocol for CD1d antibody 66257-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols