Anti-Human CD1b Rabbit Recombinant Antibody

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  FC experiment of human monocyte-derived mature dendritic cells using 98579-2-RR

  1x10^6 untreated monocytes or human monocyte-derived mature dendritic cells were surface stained with 0.25 ug Anti-Human CD1b Rabbit RecAb (98579-2-RR, Clone:251556D7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human HLA-DR (L243). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human monocyte-derived mature dendritic cells using 98579-2-RR

  1x10^6 human monocyte-derived mature dendritic cells were surface stained with 0.25 ug Anti-Human CD1B Rabbit RecAb (98579-2-RR, Clone: 251556D7) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human CD1B Rabbit RecAb (98579-2-RR, Clone: 251556D7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  FC experiment of MOLT-4 using 98579-2-RR

  1x10^6 MOLT-4 cells were surface stained with 0.25 ug Anti-Human CD1b Rabbit RecAb (98579-2-RR, Clone: 251556D7) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

CD1b Uni-rAb^® Recombinant Antibody for FC

Cat No. 98579-2-RR

Clone No.251556D7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD1, CD1b molecule, R1

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Tested Applications

• Positive FC detected in: human monocyte-derived mature dendritic cells, MOLT-4 cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98579-2-RR targets CD1b in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: CD1b molecule
• Calculated Molecular Weight: 333 aa, 37 kDa
• GenBank Accession Number: BC069481
• Gene Symbol: CD1B
• Gene ID (NCBI): 910
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P29016
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD1b is a ~45 kDa transmembrane glycoprotein that belongs to the MHC class I-like CD1 family. It associates non-covalently with β2-microglobulin to form a functional antigen-presenting heterodimer. CD1b is expressed on cortical thymocytes, dendritic cells (PMID: 17593659). It has the capacity to bind self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on T cells (PMID: 30399491).