CD2 Polyclonal antibody

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  WB analysis using 31947-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31947-1-AP (CD2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

CD2 Polyclonal Antibody for WB, ELISA

Cat No. 31947-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

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Tested Applications

• Positive WB detected in: CTLL-2 cells, mouse spleen tissue, mouse thymus tissue, rat spleen tissue, rat thymus tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31947-1-AP targets CD2 in WB, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD2 antigen
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: NM_013486.2
• Gene Symbol: CD2
• Gene ID (NCBI): 12481
• RRID: AB_3670154
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P08920
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.