Anti-Mouse CD204 Rabbit Recombinant Antibody

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  FC experiment of mouse peritoneal macrophages using 98576-2-RR

  1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse CD204 Rabbit RecAb (98576-2-RR, Clone: 251367F5) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Mouse F4/80. Cells were not fixed.

• 

  FC experiment of mouse peritoneal macrophages using 98576-2-RR

  1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse CD204 Rabbit RecAb (98576-2-RR, Clone: 251367F5) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  FC experiment of RAW 264.7 using 98576-2-RR

  1x10^6 RAW 264.7 cells were surface stained with 0.25 ug Anti-Mouse CD204 Rabbit RecAb (98576-2-RR, Clone: 251367F5) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98576-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98576-2-RR against Mouse CD204 were performed. The affinity constant is below 1 pM.

CD204 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98576-2-RR

Clone No.251367F5

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

Macrophage acetylated LDL receptor I and II, macrophage scavenger receptor 1, Macrophage scavenger receptor types I and II, Msr1, Scavenger receptor type A

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Tested Applications

• Positive FC detected in: mouse peritoneal macrophages, RAW 264.7 cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98576-2-RR targets CD204 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg4727

  Product name: Recombinant Mouse MSR1 protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-N-rFc

  Tag: N-rFc

  Domain: 79-458 aa of NM_001113326.1

  Sequence: QLLNWEMKNCLVCSLNTSDTSQGPMEKENTSKVEMRFTIIMEHMKDMEERIESISNSKADLIDTERFQNFSMATDQRLNDILLQLNSLISSVQEHGNSLDAISKSLQSLNMTLLDVQLHTETLNVRVRESTAKQQEDISKLEERVYKVSAEVQSVKEEQAHVEQEVKQEVRVLNNITNDLRLKDWEHSQTLKNITFIQGPPGPQGEKGDRGLTGQTGPPGAPGIRGIPGVKGDRGQIGFPGGRGNPGAPGKPGRSGSPGPKGQKGEKGSVGGSTPLKTVRLVGGSGAHEGRVEIFHQGQWGTICDDRWDIRAGQVVCRSLGYQEVLAVHKRAHFGQGTGPIWLNEVMCFGRESSIENCKINQWGVLSCSHSEDAGVTCTS

• Full Name: macrophage scavenger receptor 1
• Calculated Molecular Weight: 50 kDa
• GenBank Accession Number: NM_001113326.1
• Gene Symbol: Msr1
• Gene ID (NCBI): 20288
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P30204-1
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD204, also known as MSR1, belongs to the scavenger receptor family, which is mainly expressed on the surface of macrophages. CD204 plays a key inflammatory role in various pathophysiological processes, including atherosclerosis, innate and adaptive immunity, lung and liver disease, and cancer. CD204 binds to a variety of ligands, including modified low-density lipoproteins (LDL) and lipids, and is involved in endocytosis, positive regulation of cholesterol storage, and positive regulation of macrophage-derived foam cell differentiation. Studies have shown that CD204 is significantly increased in peripheral blood mononuclear cells (PBMCs) from patients with asthma and chronic obstructive pulmonary disease (COPD), which may serve as a disease marker.