Glycophorin A/CD235a Polyclonal antibody

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  IHC staining of mouse placenta using 30864-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 30864-1-AP (CD235a antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse placenta using 30864-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 30864-1-AP (CD235a antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Glycophorin A/CD235a Polyclonal Antibody for IHC, ELISA

Cat No. 30864-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IHC, ELISA

Synonyms

glycophorin A, Gypa, GPA, Glycophorin-A, CD235a

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Tested Applications

• Positive IHC detected in: mouse placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30864-1-AP targets Glycophorin A/CD235a in IHC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Glycophorin A/CD235a fusion protein Ag33770
• Full Name: glycophorin A
• Calculated Molecular Weight: 18kd
• GenBank Accession Number: NM_010369
• Gene Symbol: Gypa
• Gene ID (NCBI): 14934
• RRID: AB_3669769
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P14220
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)