TEM1 Polyclonal antibody

TEM1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 18160-1-AP

Synonyms

CD164L1, CD248, CD248 molecule, endosialin, Endosialin, TEM1, Tumor endothelial marker 1



Tested Applications

Positive WB detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18160-1-AP targets TEM1 in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TEM1 fusion protein Ag12990
Full Name CD248 molecule, endosialin
Calculated Molecular Weight 757 aa, 81 kDa
Observed Molecular Weight160 kDa
GenBank Accession NumberBC051340
Gene Symbol CD248
Gene ID (NCBI) 57124
RRIDAB_2073200
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TEM1 (Tumor endothelial marker 1), also named as CD248, Endosialin and CD164L1, is a C-type lectin-like domain (CTLD) containing type I transmembrane glycoprotein. It is now considered to be a highly selective marker for activated perivascular and stromal cells, detected in most cancers and at least some inflammatory disorders. CD248 plays a role in tumor angiogenesis. It is a potential diagnostic tool and therapeutic target of inflammatory and malignant disease. Two isoforms of human TEM1 exist. The calculated molecular weights of the two isoforms are 81 kDa and 46 kDa, respectively. Native TEM1 can be glycosylated, and the glycosylated form has a larger apparent molecular weight than 81 kDa.

Protocols

Product Specific Protocols
WB protocol for TEM1 antibody 18160-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cancer Cell

Cancer-associated fibroblast phenotypes are associated with patient outcome in non-small cell lung cancer

Authors - Lena Cords
humanIF

Cancer Res

Interaction with CD68 and regulation of GAS6 expression by endosialin in fibroblasts drives recruitment and polarization of macrophages in hepatocellular carcinoma.

Authors - Fa Yang
ratCoIP

Clin Transl Med

CD248 as a novel therapeutic target in pulmonary arterial hypertension.

Authors - Tao Xu
human,mouseWB,IHC

J Invest Dermatol

Tumor Endothelial Marker 1 (TEM1/Endosialin/CD248) Enhances Wound Healing by Interacting with Platelet-Derived Growth Factor Receptors.

Authors - Yi-Kai Hong
mouseIF

EBioMedicine

Specific loss of adipocyte CD248 improves metabolic health via reduced white adipose tissue hypoxia, fibrosis and inflammation.

Authors - Paul Petrus
humanWB,IHC

Oncotarget

Generation of a novel Antibody-Drug Conjugate targeting endosialin: potent and durable antitumor response in sarcoma.

Authors - Emily Capone