Anti-Rabbit CD27 Mouse Recombinant Antibody

• Home
• Products
• Primary Antibodies
• Flow Cytometry Antibodies
• Anti-Rabbit CD27 Mouse Recombinant Antibody (98309-8-MR)

• 

  FC experiment of rabbit splenocytes using 98309-8-MR

  1x10^6 rabbit splenocytes cells were surface stained with 0.25 ug Anti-Rabbit CD27 Mouse RecAb (98309-8-MR, Clone:250403B10) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and PE-conjugated Nano-Secondary® anti-mouse IgG2a(recombinant VHH). Cells were co-stained with CoraLite® Plus 488 Anti-Rabbit CD3. Cells were not fixed.

• 

  FC experiment of rabbit splenocytes using 98309-8-MR

  1x10^6 rabbit splenocytes cells were surface stained with 0.25 ug Anti-Rabbit CD27 Mouse RecAb (98309-8-MR, Clone:250403B10)(red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4)(blue) and PE-conjugated Nano-Secondary® anti-mouse IgG2a(recombinant VHH). Cells were co-stained with CoraLite® Plus 488 Anti-Rabbit CD3. Cells were not fixed.

• 

  Affinity of 98309-8-MR

  Biolayer interferometry (BLl) kinetic assays of 98309-8-MR against Rabbit CD27 were performed. The affinity constant is 43.6 nM.

CD27 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98309-8-MR

Clone No.250403B10

Host / Isotype

Mouse / IgG2a

Reactivity

rabbit

Applications

FC

Synonyms

CD27 antigen, CD27 molecule, CD27L receptor, T14, T-cell activation antigen CD27

---

Tested Applications

• Positive FC detected in: rabbit splenocytes cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98309-8-MR targets CD27 in FC applications and shows reactivity with rabbit samples.

• Tested Reactivity: rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2485

  Product name: recombinant rabbit CD27 protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 10007020 of

  Sequence:

• Full Name: CD27
• Gene Symbol: CD27
• Gene ID (NCBI): 100346881
• RRID: AB_3745968
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.