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B7-H3 Monoclonal antibody

B7-H3 Monoclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

CloneNo.

1E7D1

Cat no : 66481-1-Ig

Synonyms

4Ig B7 H3, B7 homolog 3, B7-H3, CD276, CD276 antigen, CD276 molecule, Costimulatory molecule, UNQ309/PRO352



Tested Applications

Positive WB detected inHEK-293 cells, A431 cells, PC-3 cells
Positive IHC detected inhuman tonsillitis tissue, human placenta tissue, human prostate cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Positive FC detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66481-1-Ig targets B7-H3 in WB, IHC, IF, FC,ELISA applications and shows reactivity with Human, Pig samples.

Tested Reactivity Human, Pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen B7-H3 fusion protein Ag6006
Full Name CD276 molecule
Calculated molecular weight 57 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC062581
Gene symbol CD276
Gene ID (NCBI) 80381
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).

Protocols

Product Specific Protocols
WB protocol for B7-H3 antibody 66481-1-IgDownload protocol
IHC protocol for B7-H3 antibody 66481-1-IgDownload protocol
IF protocol for B7-H3 antibody 66481-1-IgDownload protocol
FC protocol for B7-H3 antibody 66481-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols