CoraLite®594-conjugated B7-H3 Monoclonal antibody

B7-H3 Monoclonal Antibody for IF, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig

Applications

IF, ELISA

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E7D1

Cat no : CL594-66481

Synonyms

4Ig B7 H3, B7 H3, B7 homolog 3, B7H3, CD276, CD276 antigen, CD276 molecule, Costimulatory molecule, UNQ309/PRO352



Tested Applications

Positive IF detected inhuman tonsillitis tissue
For other applications, we recommend the unconjugated version of this antibody, 66481-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

CL594-66481 targets B7-H3 in IF, ELISA applications and shows reactivity with Human, Pig samples.

Tested Reactivity Human, Pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen B7-H3 fusion protein Ag6006
Full Name CD276 molecule
Calculated molecular weight 57 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC062581
Gene symbol CD276
Gene ID (NCBI) 80381
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).

Protocols

Product Specific Protocols
IF protocol for CL594-conjugated B7-H3 antibody CL594-66481Download protocol
Standard Protocols
Click here to view our Standard Protocols