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CoraLite®594-conjugated B7-H3 Monoclonal antibody

B7-H3 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a


Human, Pig




CoraLite®594 Fluorescent Dye



Cat no : CL594-66481


4Ig B7 H3, B7 H3, B7 homolog 3, B7H3, B7-H3, CD276, CD276 antigen, CD276 molecule, Costimulatory molecule, UNQ309/PRO352

Tested Applications

Positive IF detected inhuman tonsillitis tissue

Recommended dilution

Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

CL594-66481 targets B7-H3 in IF applications and shows reactivity with Human, Pig samples.

Tested Reactivity Human, Pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen B7-H3 fusion protein Ag6006
Full Name CD276 molecule
Calculated molecular weight 57 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC062581
Gene symbol CD276
Gene ID (NCBI) 80381
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).


Product Specific Protocols
IF protocol for CL594 B7-H3 antibody CL594-66481Download protocol
Standard Protocols
Click here to view our Standard Protocols