CoraLite® Plus 488 Anti-Mouse CD3e (145-2C11)

• 

  FC experiment of C57BL/6 mouse splenocytes using CL488-65060

  1X10^6 C57BL/6 mouse splenocytes were surface co-stained with PE Anti-Mouse CD4 (GK1.5) and 0.5 ug CoraLite® Plus 488 Anti-Mouse CD3ε (CL488-65060, Clone:145-2C11) or 0.5 ug Armenian Hamster IgG isotype Control. Cells were not fixed.

CD3e Monoclonal Antibody for FC

Cat No. CL488-65060

Clone No.145-2C11

Host / Isotype

Hamster / IgG

Reactivity

mouse

Applications

FC

Synonyms

145-2C11, AI504783, CD3, CD3epsilon, CD3ε

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65060 targets CD3e in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Hamster / IgG
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  H-2Kb-specific murine cytotoxic T-lymphocyte (CTL) clone BM10-37

• Full Name: CD3 antigen, epsilon polypeptide
• GenBank Accession Number: BC098236
• Gene Symbol: CD3ε
• Gene ID (NCBI): 12501
• RRID: AB_3084151
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P22646
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).