Tested Applications
| Positive FC detected in | mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| FC | See 9 publications below |
Product Information
FITC-65060 targets CD3e in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Hamster / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
H-2Kb-specific murine cytotoxic T-lymphocyte (CTL) clone BM10-37 Predict reactive species |
| Full Name | CD3 antigen, epsilon polypeptide |
| GenBank Accession Number | BC098236 |
| Gene Symbol | CD3ε |
| Gene ID (NCBI) | 12501 |
| RRID | AB_2883750 |
| Conjugate | FITC Plus Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 495 nm / 524 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P22646 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for FITC Plus CD3e antibody FITC-65060 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Cancer-Associated Fibroblast-Induced Remodeling of Tumor Microenvironment in Recurrent Bladder Cancer | ||
Cell Death Discov TRAF6 triggers Mycobacterium-infected host autophagy through Rab7 ubiquitination | ||
Drug Deliv Sonodynamical reversion of immunosuppressive microenvironment in prostate cancer via engineered exosomes. | ||
Asian J Pharm Sci Lignin-assisted construction of sub-10 nm supramolecular self-assembly for photothermal immunotherapy and potentiating anti-PD-1 therapy against primary and distant breast tumors | ||
J Thromb Haemost Enhanced metabolic activation of and platelet response to clopidogrel in T-cell deficient mice through induction of Cyp2c and Cyp3a and inhibition of Ces1 | ||
ACS Nano Bioengineered Nanospores Selectively Blocking LC3-Associated Phagocytosis in Tumor-Associated Macrophages Potentiate Antitumor Immunity |
