FITC Plus Anti-Mouse CD3ε (145-2C11)

CD3ε Monoclonal Antibody for FC

Host / Isotype

Hamster / IgG

Reactivity

Mouse and More (1)

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

145-2C11

Cat no : FITC-65060

Synonyms

AI504783, CD3, CD3e, CD3epsilon, CD3ε, T3e



Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

FCSee 7 publications below

Product Information

FITC-65060 targets CD3ε in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Cited Reactivitymouse
Host / Isotype Hamster / IgG
Class Monoclonal
Type Antibody
Immunogen H-2Kb-specific murine cytotoxic T-lymphocyte (CTL) clone BM10-37
Full Name CD3 antigen, epsilon polypeptide
GenBank accession numberBC098236
Gene symbol Cd3e
Gene ID (NCBI) 12501
RRIDAB_2883750
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission maxima wavelengths495 nm / 524 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Protocols

Product Specific Protocols
FC protocol for FITC Plus CD3ε antibody FITC-65060Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseFC

Adv Sci (Weinh)

Cancer-Associated Fibroblast-Induced Remodeling of Tumor Microenvironment in Recurrent Bladder Cancer

Authors - Ting Liang
mouseFC

Drug Deliv

Sonodynamical reversion of immunosuppressive microenvironment in prostate cancer via engineered exosomes.

Authors - Dingyi Wang
mouseFC

Asian J Pharm Sci

Lignin-assisted construction of sub-10 nm supramolecular self-assembly for photothermal immunotherapy and potentiating anti-PD-1 therapy against primary and distant breast tumors

Authors - Xudong Fan
mouseFC

J Thromb Haemost

Enhanced metabolic activation of and platelet response to clopidogrel in T-cell deficient mice through induction of Cyp2c and Cyp3a and inhibition of Ces1

Authors - Li-Ping Jiang
mouseFC

ACS Nano

Bioengineered Nanospores Selectively Blocking LC3-Associated Phagocytosis in Tumor-Associated Macrophages Potentiate Antitumor Immunity

Authors - Yunkai Shang
mouseFC

Vet Microbiol

A self-assembled nanoparticle vaccine based on pseudorabies virus glycoprotein D induces potent protective immunity against pseudorabies virus infection

Authors - Xujiao Ren