CD315/PTGFRN Polyclonal antibody

• 

  WB analysis using 33875-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33875-1-AP (CD315/PTGFRN antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

CD315/PTGFRN Polyclonal Antibody for WB, ELISA

Cat No. 33875-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

CD315, CD9 partner 1, CD9P-1, EWI-F, Fprp

---

Tested Applications

• Positive WB detected in: A549 cells, C2C12 cells, PC-12 cells, mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33875-1-AP targets CD315/PTGFRN in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Eg6319

  Product name: Recombinant mouse CD315/PTGFRN protein (C-rFc)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 22-832 aa of NM_011197.3

  Sequence: RVVRVPAGTLVRVVGTELVIPCNVSDYDGPSEQNFDWSFSSSGSSFVELASTWEVGFPAQLYRERLQRGDILLRRTANDAVELHIKNVQPSDQGHYKCSTPSTDATVQGNYEDTVQVKVLADALVVGPSSRPPPGLSLREGEPFELRCIASTTSPLHTHLALRWELHRGPVHRSILALSHEGRFHPGPGYEQRYHSGDVRLDTVGSDAYRLSVARALSADQGSYRCVVSEWITEQGSWQEIQEKAVEVATVVIQPTALQLAVPRTVSVTEGKDLDLSCNITTDRVDDVRPEVTWYFKKTPDTSLLASHMLARLDRDSLVHSSPHVALSHVDTRSYHLLVRDVSKENSGYYLCLVALWAPGHNRSWHKVAEAMSAPSGVSVTWLEPEYQVYLNASKVPGFSDDPTELQCRVIDTKRLEAGVRLTVSWYYRMTRRNDDVVASELLAVMDGDWTLRYGERSKQRAQDGEFIFSKEHTDTFNFRIQRTTEEDRGNYYCVVSAWTRQRNNSWVKSKDVFSKPVNIFWASEDSVLVVKARQPKPFFAAGNTFEMTCKVSSKNIKSPRYSVLITAEKPVGDLSSPNETKYIISLDQDSVVKLENWTDASRVDGVVLEKVQEDEFRYRMYQTQVSDAGLYRCMVTAWSPIGGSLWREAATSLSNPIEIDFQTSGPTFNASVHSDTPSVTRGDLIKLFCIVTVEGAVLDPDDMAFDVSWFAVHSFGLDKAPVLLSSLDRKGVVTTGQRDWKSTVSLERVSVLEFLLQVHGSEDQDFGNYYCSVTPWVRSPTGSWQREAEIHSRPIFITVKMDVLNAFKYP

• Full Name: prostaglandin F2 receptor negative regulator
• Calculated Molecular Weight: 99 kDa
• Observed Molecular Weight: 100-130 kDa
• GenBank Accession Number: NM_011197.3
• Gene Symbol: Ptgfrn
• Gene ID (NCBI): 19221
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9WV91
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTGFRN (Prostaglandin F2 Receptor Negative Regulator), also known as CD315, is a trans-membrane protein that modulates prostaglandin F2α signaling and links the tetraspanin web to the cytoskeleton, it is now recognized as a driver of metastasis; high expression correlates with aggressive proliferation, migration, and spheroid growth in epidermoid carcinoma, medulloblastoma, and mesothelioma, while silencing PTGFRN impairs these traits and disrupts autophagy (PMID: 38924562). The calculated value of PTGFRN is 98 kDa. It has multiple glycosylation sites and its molecular weight after glycosylation is approximately 100 - 130 kDa.