CoraLite® Plus 488 Anti-Mouse CD4 (GK1.5)
CD4 Monoclonal Antibody for IF/ICC, FC
Host / Isotype
Rat / IgG2b, kappa
Reactivity
Mouse
Applications
IF/ICC, FC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
GK1.5
Cat no : CL488-65104
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | mouse splenocytes |
Positive FC detected in | mouse splenocytes |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-65104 targets CD4 in IF/ICC, FC applications and shows reactivity with Mouse samples.
Tested Reactivity | Mouse |
Host / Isotype | Rat / IgG2b, kappa |
Class | Monoclonal |
Type | Antibody |
Immunogen | Mouse CTL clone V4 |
Full Name | CD4 antigen |
GenBank Accession Number | BC039137 |
Gene Symbol | Cd4 |
Gene ID (NCBI) | 12504 |
RRID | AB_2883178 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Affinity purification |
Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 488 CD4 antibody CL488-65104 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |