CoraLite® Plus 488 Anti-Mouse CD4 (GK1.5)

CD4 Monoclonal Antibody for IF/ICC, FC
Cat No. CL488-65104
Clone No.GK1.5

Host / Isotype

Rat / IgG2b, kappa

Reactivity

mouse

Applications

IF/ICC, FC

Ly 4, L3T4, GK1.5, CD4 antigen

Formulation:  PBS and Azide
PBS and Azide
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive IF/ICC detected inmouse splenocytes
Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

FCSee 1 publications below

Product Information

CL488-65104 targets CD4 in IF/ICC, FC applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Cited Reactivitymouse
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen Mouse CTL clone V4 Predict reactive species
Full Name CD4 antigen
GenBank Accession NumberBC039137
Gene Symbol Cd4
Gene ID (NCBI) 12504
RRIDAB_2883178
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP06332
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CD4 antibody CL488-65104Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseFC

Acta Pharm Sin B

Novel Pt(IV) complex OAP2 induces STING activation and pyroptosis via mitochondrial membrane remodeling for synergistic chemo-immunotherapy

Authors - Renming Fan
Loading...