CoraLite®647 Anti-Mouse CD4 (GK1.5)

CD4 Monoclonal Antibody for IF,FC

Host / Isotype

Rat / IgG2b

Reactivity

Mouse

Applications

IF,FC

Conjugate

CoraLite®647 Fluorescent Dye

CloneNo.

GK1.5

Cat no : CL647-65104

Synonyms

Cd4, CD4 antigen, L3T4, Ly 4



Tested Applications

Positive IF detected inmouse splenocytes
Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65104-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:250-1:1000
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL647-65104 targets CD4 in IF,FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2b
Class Monoclonal
Type Antibody
Immunogen Mouse CTL clone V4
Full Name CD4 antigen
GenBank accession numberBC039137
Gene symbol Cd4
Gene ID (NCBI) 12504
Conjugate CoraLite®647 Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm/665 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Protocols

Product Specific Protocols
IF protocol for CL647 CD4 antibody CL647-65104Download protocol
FC protocol for CL647 CD4 antibody CL647-65104Download protocol
Standard Protocols
Click here to view our Standard Protocols