CD40 Polyclonal antibody

• Featured Product
• KD/KO Validated

CD40 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 28158-1-AP

Synonyms

TNFRSF5, p50, CD40L receptor, Bp50, B-cell surface antigen CD40

---

Validation Data Gallery

• 

  WB analysis using 28158-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28158-1-AP (CD40 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 28158-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28158-1-AP (CD40 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 28158-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28158-1-AP (CD40 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Daudi using 28158-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Daudi cells using CD40 antibody (28158-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

---

Tested Applications

• Positive WB detected in: Daudi cells, Raji cells, Ramos cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Daudi cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Product Information

28158-1-AP targets CD40 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD40 fusion protein Ag28033
• Full Name: CD40 molecule, TNF receptor superfamily member 5
• Calculated Molecular Weight: 277 aa, 31 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC012419
• Gene Symbol: CD40
• Gene ID (NCBI): 958
• RRID: AB_2881078
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cluster of differentiation 40 (CD40) is a costimulatory protein located on antigen presenting cells and is required for their activation. CD40 is a member of the tumor necrosis factor (TNF) receptor (TNFR) family.

What is the molecular weight of CD40?

The molecular weight of CD40 is 43 kDa.

What is the cellular localization of CD40?

CD40 can be secreted by cells or found in the cell membrane.

What is the tissue specificity of CD40?

CD40 is expressed in B cells and primary carcinoma cells but is also found in dendritic cells and macrophages (PMID: 10209159).

What is the function of CD40?

CD40 acts as a receptor for TNFSF5/CD40LG, which is expressed on activated T cells. This interaction is essential for B cell proliferation, expression of activation markers, immunoglobulin production, and isotype switching (PMID: 8809473). This interaction is also crucial for the formation of memory B cells and germinal centers, and signaling through CD40 prevents apoptosis of germinal center B cells.

What is the role of CD40 in disease?

Defects in CD40 lead to hyper-IgM immunodeficiency syndrome type 3 (HIGM3) (PMID: 11675497). This is an autosomal recessive disorder that includes the inability of B cells to undergo isotype switching, a key step in the final differentiation of the humoral immune response, and an inability to mount an antibody-specific immune response.

Publications

Species	Application	Title
human	IHC	J Cell Mol Med; Machine learning-based identification of a cell death-related signature associated with prognosis and immune infiltration in glioma; Authors - Quanwei Zhou
human	WB	Toxicology; Role of miR-145-5p/ CD40 in the inflammation and apoptosis of HUVECs induced by PM2.5.; Authors - Xueting Zhang
human	WB	Cardiovasc Ther; Bazedoxifene Plays a Protective Role against Inflammatory Injury of Endothelial Cells by Targeting CD40.; Authors - Wenmin Song ; KD Validated
human	WB	FEBS Open Bio; A methylation-driven gene panel predicts survival in patients with colon cancer.; Authors - Yaojun Peng
human	IHC	Front Immunol; TANK shapes an immunosuppressive microenvironment and predicts prognosis and therapeutic response in glioma; Authors - Shasha Li
human	WB	Adv Sci (Weinh); Irisin-Encapsulated Mitochondria-Targeted Biomimetic Nanotherapeutics for Alleviating Acute Kidney Injury; Authors - Xia Zhang