CD41/Integrin Alpha 2B Monoclonal antibody

CD41/Integrin Alpha 2B Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

6D7C5

Cat no : 60350-1-Ig

Synonyms

CD41, CD41/Integrin alpha 2b, CD41B, GP2B, GPalpha IIb, GPIIb, GTA, HPA3, Integrin alpha IIb, ITGA2B, ITGAB

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Validation Data Gallery

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  WB analysis of human spleen using 60350-1-Ig

  human spleen tissue were subjected to SDS PAGE followed by western blot with 60350-1-Ig (CD41/Integrin alpha 2b Antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3200

Sample-dependent, check data in validation data gallery

Product Information

60350-1-Ig targets CD41/Integrin Alpha 2B in WB,ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41)
• Calculated molecular weight: 113 kDa
• Observed molecular weight: 125 kDa
• GenBank accession number: BC126442
• Gene symbol: ITGA2B
• Gene ID (NCBI): 3674
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha-IIb (ITGA2B, CD41) is expressed on platelets, megakaryocytes and some hematopoietic progenitor cells (PMID: 11934866). This protein undergoes post-translational cleavage to yield disulfide linked light (25 kDa) and heavy (125 kDa) chains which join with integrin beta3 (CD61) to form a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrin alpha-IIb/beta-3 is crucial for platelet aggregation through binding of soluble fibrinogen.