PE Anti-Human CD42b (AK2)

CD42b Monoclonal Antibody for FC

Host / Isotype

Mouse / Mouse IgG1

Reactivity

Human

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

AK2

Cat no : PE-65163

Synonyms

BSS, CD42B, CD42b alpha, GP1B, GP1BA, GPIb alpha, GPIbα



Tested Applications

Positive FC detected inhuman peripheral blood platelets

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

PE-65163 targets CD42b in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / Mouse IgG1
Class Monoclonal
Type Antibody
Immunogen Human platelets
Full Name glycoprotein Ib (platelet), alpha polypeptide
Calculated molecular weight 626 aa, 69 kDa
GenBank accession numberBC027955
Gene symbol GP1BA
Gene ID (NCBI) 2811
Conjugate PE Fluorescent Dye
Excitation/Emission maxima wavelengths496, 565 nm/578 nm
Form Liquid
Purification Method The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

CD42b, also known as platelet glycoprotein Ib alpha chain (GPIb alpha), is a type I transmembrane glycoprotein of 135-145 kDa (PMID: 2656709). It is expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are linked by a disulphide bond to form GPIb (PMID: 7660135). GPIb forms a noncovalent complex with CD42a (GPIX) and CD42d (GPV) and acts as a receptor for von Willebrand factor (vWF) and thrombin (PMID: 7660135; 3759960). The GPIb-V-IX complex mediates vWF-dependent platelet adhesion to blood vessels. Defects in the expression of CD42b result in Bernard-Soulier syndromes and platelet-type von Willebrand disease (PMID: 9371310).

Protocols

Product Specific Protocols
FC protocol for PE CD42b antibody PE-65163Download protocol
Standard Protocols
Click here to view our Standard Protocols