PE Anti-Human CD42b (AK2)

CD42b Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

AK2

Cat no : PE-65163

Synonyms

AK2, BSS, CD42B, CD42b alpha, GP1B, GP1BA, GPIb alpha, GPIbα

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Validation Data Gallery

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  FC experiment of human peripheral blood platelets using PE-65163

  1X10^6 human peripheral blood platelets were surface co-stained with Anti-Human CD41a labeled with FlexAble CoraLite® Plus750 Antibody Labeling Kit for Mouse IgG1 (KFA024) and PE Anti-Human CD42b (PE-65163, Clone: AK2) or Mouse IgG1 Isotype Control. Cells were not fixed.

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Tested Applications

• Positive FC detected in: human peripheral blood platelets

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65163 targets CD42b in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human platelets
• Full Name: glycoprotein Ib (platelet), alpha polypeptide
• Calculated Molecular Weight: 626 aa, 69 kDa
• GenBank Accession Number: BC027955
• Gene Symbol: GP1BA
• Gene ID (NCBI): 2811
• RRID: AB_2883941
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD42b, also known as platelet glycoprotein Ib alpha chain (GPIb alpha), is a type I transmembrane glycoprotein of 135-145 kDa (PMID: 2656709). It is expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are linked by a disulphide bond to form GPIb (PMID: 7660135). GPIb forms a noncovalent complex with CD42a (GPIX) and CD42d (GPV) and acts as a receptor for von Willebrand factor (vWF) and thrombin (PMID: 7660135; 3759960). The GPIb-V-IX complex mediates vWF-dependent platelet adhesion to blood vessels. Defects in the expression of CD42b result in Bernard-Soulier syndromes and platelet-type von Willebrand disease (PMID: 9371310).