CoraLite® Plus 594 Anti-Human CD42b (AK2)

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  FC experiment of human peripheral blood platelets using CL594-65163

  100ul human platelet-rich plasma (diluted 1:2 in PBS) was surface stained with 5 ul CoraLite® Plus 594 Anti-Human CD42b (CL594-65163, Clone: AK2) or CoraLite®594 Mouse IgG1 Isotype Control (CL594-65124). Cells were not fixed.

• 

  FC experiment of human peripheral blood platelets using CL594-65163

  100ul human platelet-rich plasma (diluted 1:2 in PBS) was surface stained with 5 ul CoraLite® Plus 594 Anti-Human CD42b (CL594-65163, Clone: AK2) or CoraLite®594 Mouse IgG1 Isotype Control (CL594-65124). Cells were not fixed.

CD42b Monoclonal Antibody for FC

Cat No. CL594-65163

Clone No.AK2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC

Synonyms

GP1BA, GP1B, CD42b alpha, BSS, Antigen CD42b-alpha

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Tested Applications

• Positive FC detected in: human peripheral blood platelets

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-65163 targets CD42b in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Human platelets

• Full Name: glycoprotein Ib (platelet), alpha polypeptide
• Calculated Molecular Weight: 626 aa, 69 kDa
• GenBank Accession Number: BC027955
• Gene Symbol: CD42b
• Gene ID (NCBI): 2811
• ENSEMBL Gene ID: ENSG00000185245
• Conjugate: CoraLite® Plus 594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 594 nm / 615 nm
• Form: Liquid
• Purification Method: Purified by protein-A affinity chromatography
• UNIPROT ID: P07359
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD42b, also known as platelet glycoprotein Ib alpha chain (GPIb alpha), is a type I transmembrane glycoprotein of 135-145 kDa (PMID: 2656709). It is expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are linked by a disulphide bond to form GPIb (PMID: 7660135). GPIb forms a noncovalent complex with CD42a (GPIX) and CD42d (GPV) and acts as a receptor for von Willebrand factor (vWF) and thrombin (PMID: 7660135; 3759960). The GPIb-V-IX complex mediates vWF-dependent platelet adhesion to blood vessels. Defects in the expression of CD42b result in Bernard-Soulier syndromes and platelet-type von Willebrand disease (PMID: 9371310).