Anti-Human CD42b (AK2)

CD42b Monoclonal Antibody for FC

Host / Isotype

Mouse / Mouse IgG1

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

AK2

Cat no : 65163-1-Ig

Synonyms

BSS, CD42B, CD42b alpha, GP1B, GP1BA, GPIb alpha, GPIbα



Tested Applications

Positive FC detected inHuman peripheral blood platelets

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

65163-1-Ig targets CD42b in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / Mouse IgG1
Class Monoclonal
Type Antibody
Immunogen Human platelets
Full Name glycoprotein Ib (platelet), alpha polypeptide
Calculated molecular weight 626 aa, 69 kDa
GenBank accession numberBC027955
Gene symbol GP1BA
Gene ID (NCBI) 2811
Conjugate Unconjugated
Form Liquid
Purification Method Purified by protein-A affinity chromatography
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C.

Background Information

CD42b, also known as platelet glycoprotein Ib alpha chain (GPIb alpha), is a type I transmembrane glycoprotein of 135-145 kDa (PMID: 2656709). It is expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are linked by a disulphide bond to form GPIb (PMID: 7660135). GPIb forms a noncovalent complex with CD42a (GPIX) and CD42d (GPV) and acts as a receptor for von Willebrand factor (vWF) and thrombin (PMID: 7660135; 3759960). The GPIb-V-IX complex mediates vWF-dependent platelet adhesion to blood vessels. Defects in the expression of CD42b result in Bernard-Soulier syndromes and platelet-type von Willebrand disease (PMID: 9371310).

Protocols

Product Specific Protocols
FC protocol for CD42b antibody 65163-1-IgDownload protocol
Standard Protocols
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