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- KD/KO Validated
CD44 Monoclonal antibody
CD44 Monoclonal Antibody for FC, IF, IHC, WB,ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, pig
Applications
WB, IHC, IF, FC,ELISA
Conjugate
Unconjugated
CloneNo.
6F4H2
Cat no : 60224-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HUVEC cells, human spleen tissue, pig spleen tissue, A549 cells, MDA-MB-231 cells |
| Positive IHC detected in | human lung cancer tissue, human breast cancer tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human lung cancer tissue |
| Positive FC detected in | Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 7 publications below |
| IHC | See 2 publications below |
| IF | See 6 publications below |
| FC | See 1 publications below |
Product Information
The immunogen of 60224-1-Ig is CD44 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, pig |
| Cited Reactivity | human, pig |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CD44 fusion protein Ag7633 |
| Full Name | CD44 molecule (Indian blood group) |
| Calculated molecular weight | 742 aa, 82 kDa |
| Observed molecular weight | 80-95 kDa |
| GenBank accession number | BC004372 |
| Gene symbol | CD44 |
| Gene ID (NCBI) | 960 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD44 antibody 60224-1-Ig | Download protocol |
| IHC protocol for CD44 antibody 60224-1-Ig | Download protocol |
| IF protocol for CD44 antibody 60224-1-Ig | Download protocol |
| FC protocol for CD44 antibody 60224-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Mater Technol The Unusual Properties of Polytetrafluoroethylene Enable Massive-Volume Vitrification of Stem Cells with Low-Concentration Cryoprotectants. | ||
Adv Sci (Weinh) A Novel Long Non-Coding RNA lnc030 Maintains Breast Cancer Stem Cell Stemness by Stabilizing SQLE mRNA and Increasing Cholesterol Synthesis. | ||
Aging (Albany NY) Identification of a mesenchymal-related signature associated with clinical prognosis in glioma. | ||
Cell Reprogram Inhibition of DNA Methyltransferase by RG108 Promotes Pluripotency-Related Character of Porcine Bone Marrow Mesenchymal Stem Cells. | ||
J Reprod Dev Hyaluronan improves neither the long-term storage nor the cryosurvival of liquid-stored CD44-bearing AI boar spermatozoa. |
