CD44 Monoclonal antibody

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  WB analysis using 60224-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60224-1-Ig (CD44 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

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  WB analysis using 60224-1-Ig

  HeLa (CD44+), A549 (CD44+), MDA-MB-231 (CD44+), MCF-7, Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 60224-1-Ig (CD44 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa cells using 60224-1-Ig

  WB result of CD44 antibody (60224-1-Ig, 1:4000) with si-Control and si-CD44 transfected HeLa cells.

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  WB analysis of human spleen using 60224-1-Ig

  human spleen tissue were subjected to SDS PAGE followed by western blot with 60224-1-Ig (CD44 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 60224-1-Ig

  IP result of anti-CD44 (IP:60224-1-Ig, 4ug; Detection:60224-1-Ig 1:3000) with HeLa cells lysate 1280 ug.

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  IHC staining of human lung cancer using 60224-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 60224-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 60224-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60224-1-Ig (CD44 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 60224-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60224-1-Ig (CD44 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 60224-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60224-1-Ig (CD44 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 60224-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 60224-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 60224-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 60224-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human colon cancer using 60224-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using CD44 antibody (60224-1-Ig, Clone: 6F4H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human colon cancer using 60224-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using CD44 antibody (60224-1-Ig, Clone: 6F4H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 60224-1-Ig

  Immunofluorescent analysis of un-fixed A431 cells using CD44 antibody (60224-1-Ig, Clone: 6F4H2 ) at dilution of 1:1800 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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  IF Staining of A431 using 60224-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CD44 antibody (60224-1-Ig, Clone: 6F4H2 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

• Featured Product
• KD/KO Validated

CD44 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 60224-1-Ig

Clone No.6F4H2

Publications(71)

Reviews (1)

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig and More (2)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Synonyms

6F4H2, CD44 antigen, CDW44, CSPG8, ECMR III

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Tested Applications

• Positive WB detected in: MDA-MB-231 cells, human spleen tissue, HeLa cells, A549 cells, U-251 cells, HUVEC cells, pig spleen tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lung cancer tissue, human breast cancer tissue, human oesophagus cancer tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human colon cancer tissue
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 37 publications below
• IHC: See 10 publications below
• IF: See 33 publications below
• ELISA: See 1 publications below
• FC: See 2 publications below

Product Information

60224-1-Ig targets CD44 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Cited Reactivity: human, rat, pig, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD44 fusion protein Ag7633
• Full Name: CD44 molecule (Indian blood group)
• Calculated Molecular Weight: 742 aa, 82 kDa
• Observed Molecular Weight: 80-95 kDa
• GenBank Accession Number: BC004372
• Gene Symbol: CD44
• Gene ID (NCBI): 960
• ENSEMBL Gene ID: ENSG00000026508
• RRID: AB_11042767
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P16070
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications.

Publications

Species	Application	Title
	IF	Nature; Cell transcriptomic atlas of the non-human primate Macaca fascicularis.; Authors - Lei Han
human	IF	Nat Methods; Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM).; Authors - Marine H Laporte
		Clin Transl Med; PADI4 facilitates stem-like properties and cisplatin resistance through upregulating PRMT2/IDs family in oesophageal squamous cell carcinoma; Authors - Zeyu Wang
pig	IF	Int J Biol Macromol; A light-cured injectable composite hydrogel based on chitosan and decellularized matrix modulates stem cell aggregation behavior for accelerating cartilage defect repair; Authors - Peipei Feng
human	IF	Biomater Sci; Magnetothermal heating facilitates the cryogenic recovery of stem cell-laden alginate-Fe(3)O(4) nanocomposite hydrogels; Authors - Xiaozhang Zhang
human	IF	Biomater Sci; Vitrification of stem cell-laden core-shell microfibers with unusually low concentrations of cryoprotective agents; Authors - Conghui Tian

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Udesh (Verified Customer) (08-16-2023)

Worked well for WB at 1:1000

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Glioblastoma cells