Validation Data Gallery
|Positive WB detected in||HeLa cells, HUVEC cells, human spleen tissue, pig spleen tissue, A549 cells, MDA-MB-231 cells|
|Positive IHC detected in||human lung cancer tissue, human breast cancer tissue, human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human lung cancer tissue|
|Positive FC detected in||Raji cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 60224-1-Ig is CD44 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, pig|
|Cited Reactivity||human, pig|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||CD44 fusion protein Ag7633|
|Full Name||CD44 molecule (Indian blood group)|
|Calculated molecular weight||742 aa, 82 kDa|
|Observed molecular weight||80-95 kDa|
|GenBank accession number||BC004372|
|Gene ID (NCBI)||960|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications.
|Product Specific Protocols|
|WB protocol for CD44 antibody 60224-1-Ig||Download protocol|
|IHC protocol for CD44 antibody 60224-1-Ig||Download protocol|
|IF protocol for CD44 antibody 60224-1-Ig||Download protocol|
|FC protocol for CD44 antibody 60224-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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