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CD44 Monoclonal antibody
CD44 Monoclonal Antibody for FC, IF, IHC, WB,ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, pig and More (3)
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
6F4H2
Cat no : 60224-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HUVEC cells, human spleen tissue, pig spleen tissue, A549 cells, MDA-MB-231 cells |
Positive IHC detected in | human lung cancer tissue, human breast cancer tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | human lung cancer tissue |
Positive FC detected in | Raji cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 18 publications below |
IHC | See 4 publications below |
IF | See 16 publications below |
FC | See 1 publications below |
Product Information
The immunogen of 60224-1-Ig is CD44 Fusion Protein expressed in E. coli.
Tested Reactivity | human, pig |
Cited Reactivity | human, mouse, rat, pig, rabbit |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | CD44 fusion protein Ag7633 |
Full Name | CD44 molecule (Indian blood group) |
Calculated molecular weight | 742 aa, 82 kDa |
Observed molecular weight | 80-95 kDa |
GenBank accession number | BC004372 |
Gene symbol | CD44 |
Gene ID (NCBI) | 960 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CD44 antibody 60224-1-Ig | Download protocol |
IHC protocol for CD44 antibody 60224-1-Ig | Download protocol |
IF protocol for CD44 antibody 60224-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Methods Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM). | ||
Biomater Sci Magnetothermal heating facilitates the cryogenic recovery of stem cell-laden alginate-Fe(3)O(4) nanocomposite hydrogels | ||
Biomater Sci Vitrification of stem cell-laden core-shell microfibers with unusually low concentrations of cryoprotective agents | ||
J Nanobiotechnology Bone-targeted erythrocyte-cancer hybrid membrane-camouflaged nanoparticles for enhancing photothermal and hypoxia-activated chemotherapy of bone invasion by OSCC. | ||
Stem Cell Res Ther Glioma-associated mesenchymal stem cells-mediated PD-L1 expression is attenuated by Ad5-Ki67/IL-15 in GBM treatment. |