CD44 Fusion Protein
e coli.-derived, PET28a
Cat no : Ag7633
CDW44; CSPG8; ECMR-III; HCELL; IN; LHR; MC56; MDU2; MDU3; MGC10468; MIC4; MUTCH-I; Pgp1
(28-380 aa encoded by BC004372)
|Endotoxin Level||Please contact the lab for more information.|
|Purity||85%, by SDS-PAGE with Coomassie Brilliant Blue staining.|
|Formulation||The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.|
Reconstitution and Storage
Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage (see Stability and Storage for more details).
If a different concentration is needed for your purposes please adjust the reconstitution volume as required (please note: the ion concentration of the final solution will vary according to the volume used).
Note: Centrifuge vial before opening. When reconstituting, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution.
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature (see below).|
|Stability and Storage||Store for up to 12 months at -20°C to -80°C as lyophilized powder.|
|Storage of Reconstituted Protein||
Short-term storage: Store at 2-8°C for (1-2 weeks).
Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
New monoclonal antibodies that recognize an unglycosylated, conserved, extracellular region of CD44 in vitro and in vivo, and can block tumorigenesis.
Exosomes in specific fractions of the boar ejaculate contain CD44: A marker for epididymosomes?
Reprod Domest Anim
The risk of using monoclonal or polyclonal commercial antibodies: controversial results on porcine sperm CD44 receptor identification.