CD45 Polyclonal antibody

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• CD45 Polyclonal antibody (33651-1-AP)

• 

  WB analysis using 33651-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33651-1-AP (CD45 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat spleen using 33651-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 33651-1-AP (CD45 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat spleen using 33651-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 33651-1-AP (CD45 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD45 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 33651-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, IHC, ELISA

Synonyms

Lca, Ptprc, L CA, RT7, T200

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Tested Applications

• Positive WB detected in: NR8383 cells, rat thymus tissue
• Positive IHC detected in: rat spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

33651-1-AP targets CD45 in WB, IHC, ELISA applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Eg4666

  Product name: Recombinant Rat CD45/PTPRC protein (rFc Tag)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 24-546 aa of NM_138507.3

  Sequence: QGTTDDGLDTTEIVLLPQTDPLPARTTEFTPPSISERGNGSSETTYLPGFSSTLMPHLTPQPDSQTPSARGADTQTLSSQADLTTLTAAPSGETDPPGVPEESTVPETFPGGTPILARNSTAPSPTHTSNVSTTDISSGANLTTPAPSTLGFASNTTTSTEIATPQTKPSCDEKFGNVTVRYIYDDSSKNFNANLEGDKKPKCEYTDCEKELKNLPECSQKNVTLSNGSCTPDKIINLDVPPGTHNFNLTNCTPDIEANTSICLEWKIKNKFTCDIQKISYNFRCTPEMKTFALDKHGTLWLHNLTVRTNYTCAAEVLYNNVILLKQDRRVQTDFGTPEMLPHVQCKNSTNSTTLVSWAEPASKHHGYILCYKKTPSEKCENLANDVNSFEVKNLRPYTEYTVSLFAYVIGRVQRNGPAKDCNFRTKAARPGKVNGMKTSRASDNSINVTCNSPYEINGPEARYILEVKSGGSLVKTFNQSTCKFVVDNLYYSTDYEFLVYFYNGEYLGDPEIKPQSTSYNSK

• Full Name: protein tyrosine phosphatase, receptor type, C
• Calculated Molecular Weight: 143 kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: NM_138507.3
• Gene Symbol: CD45
• Gene ID (NCBI): 24699
• RRID: AB_3743038
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P04157-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	IHC	ACS Appl Mater Interfaces; Gold-Decorated Upconversion Luminescent Nanozyme Enables ROS-Responsive Antioxidant for Myocardial Injury Repair.; Authors - Yuemei Li