CoraLite®488 Anti-Human CD45RO (UCHL1)

CD45RO Monoclonal Antibody for IF,FC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF,FC

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

UCHL1

Cat no : CL488-65150

Synonyms

B220, CD45, CD45R, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200



Tested Applications

Positive IF detected inhuman peripheral blood mononuclear cells
Positive FC detected inhuman peripheral blood lymphocytes
For other applications, we recommend the unconjugated version of this antibody, 65150-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:150-1:600
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL488-65150 targets CD45RO in IF,FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen IL-2 dependent T cell line, CA1
Full Name protein tyrosine phosphatase, receptor type, C
GenBank accession numberBC014239
Gene symbol PTPRC
Gene ID (NCBI) 5788
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RO is the shortest CD45 isoform (lacking all three exons) and is expressed by activated and memory T cells, thymocytes, some B cells, and weakly on granulocytes and macrophage (PMID: 27100180; 12414720).

Protocols

Product Specific Protocols
IF protocol for CL488 CD45RO antibody CL488-65150Download protocol
FC protocol for CL488 CD45RO antibody CL488-65150Download protocol
Standard Protocols
Click here to view our Standard Protocols