Anti-Human CD45RO (UCHL1)

CD45RO Monoclonal Antibody for FC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC, ELISA

Conjugate

Unconjugated

CloneNo.

UCHL1

Cat no : 65150-1-Ig

Synonyms

B220, CD45, CD45R, CD45RO, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200



Tested Applications

Positive FC detected inHuman peripheral blood lymphocytes
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

65150-1-Ig targets CD45RO in FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen IL-2 dependent T cell line, CA1
Full Name protein tyrosine phosphatase, receptor type, C
GenBank accession numberBC014239
Gene symbol PTPRC
Gene ID (NCBI) 5788
Conjugate Unconjugated
Form Liquid
Purification Method Affinity purification
Storage Buffer Phosphate based buffer with 0.09% sodium azide, pH 7.2.
Storage ConditionsStore at 2-8°C.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RO is the shortest CD45 isoform (lacking all three exons) and is expressed by activated and memory T cells, thymocytes, some B cells, and weakly on granulocytes and macrophage (PMID: 27100180; 12414720).

Protocols

Product Specific Protocols
FC protocol for CD45RO antibody 65150-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols