CoraLite® Plus 488-conjugated CD47 Polyclonal antibody

CD47 Polyclonal Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-29555

Synonyms

CD47, CD47 molecule, IAP, Integrin associated protein, Leukocyte surface antigen CD47, MER6, OA3, Protein MER6

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Validation Data Gallery

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  FC experiment of HUVEC using CL488-29555

  1X10^6 HUVEC cells were surface stained with 0.25 ug CoraLite® Plus 488 Anti-Human CD47 (CL488-29555) (red), or 0.25 ug Control Antibody. Cells were not fixed .

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Tested Applications

• Positive FC detected in: HUVEC cells

Recommended dilution

Application	Dilution
Sample-dependent, check data in validation data gallery

Product Information

CL488-29555 targets CD47 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: fusion protein
• Full Name: CD47 molecule
• Calculated molecular weight: 323 aa, 35 kDa
• GenBank accession number: BC010016
• Gene symbol: CD47
• Gene ID (NCBI): 961
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.