CoraLite® Plus 488-conjugated CD47 Polyclonal antibody

CD47 Polyclonal Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-29555

Synonyms

CD47, CD47 molecule, IAP, Integrin associated protein, Leukocyte surface antigen CD47, MER6, OA3, Protein MER6



Tested Applications

Positive FC detected inHUVEC cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-29555 targets CD47 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen fusion protein
Full Name CD47 molecule
Calculated Molecular Weight 323 aa, 35 kDa
GenBank Accession NumberBC010016
Gene Symbol CD47
Gene ID (NCBI) 961
RRIDAB_2919215
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CD47 antibody CL488-29555Download protocol
Standard Protocols
Click here to view our Standard Protocols