CD5 Polyclonal antibody, PBS Only

• 

  WB analysis using 31502-1-AP (same clone as 31502-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31502-1-AP (CD5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31502-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 31502-1-AP (same clone as 31502-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 31502-1-AP (CD5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31502-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 31502-1-AP (same clone as 31502-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 31502-1-AP (CD5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31502-1-PBS in a different storage buffer formulation.

CD5 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 31502-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

31502-1-PBS targets CD5 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: CD5 molecule
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: NM_014207.4
• Gene Symbol: CD5
• Gene ID (NCBI): 921
• ENSEMBL Gene ID: ENSG00000110448
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P06127
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).