Anti-Human ICAM-1/CD54 (15.2) Rabbit IgG Recombinant Antibody, PBS Only

• 

  FC experiment of human PBMCs using 65509-1-RR (same clone as 65509-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.05 ug Anti-Human CD54 Rabbit Recombinant Antibody (65509-1-RR, Clone:15.2) or Rabbit IgG control Rabbit PolyAb, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65509-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65509-1-PBS

  1x10^6 human PBMCs were surface stained with 0.05 ug Anti-Human CD54 Rabbit Recombinant Antibody (65509-1-PBS, Clone:15.2) or Rabbit IgG control Rabbit PolyAb, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using 65509-1-RR (same clone as 65509-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.05 ug Anti-Human CD54 Rabbit Recombinant Antibody (65509-1-RR, Clone:15.2) or Rabbit IgG control Rabbit PolyAb, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65509-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65509-1-PBS

  1x10^6 human PBMCs were surface stained with 0.05 ug Anti-Human CD54 Rabbit Recombinant Antibody (65509-1-PBS, Clone:15.2) or Rabbit IgG control Rabbit PolyAb, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using 65509-1-PBS

  1x10^6 human PBMCs were surface stained with 0.05 ug Anti-Human CD54 Rabbit Recombinant Antibody (65509-1-PBS, Clone:15.2) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000 (left and red), or stained with 0.05 ug Anti-Human CD54 Mouse Monoclonal Antibody (Clone:15.2) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000 (center and green). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using 65509-1-RR (same clone as 65509-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.05 ug Anti-Human CD54 Rabbit Recombinant Antibody (65509-1-RR, Clone:15.2) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Rabbit IgG(H+L) at dilution 1:1000 (left and red), or stained with 0.05 ug Anti-Human CD54 Mouse Monoclonal Antibody (Clone:15.2) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000 (center and green). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65509-1-PBS in a different storage buffer formulation.

ICAM-1/CD54 Uni-rAb^® Recombinant Antibody for FC

Cat No. 65509-1-PBS

Clone No.15.2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD54, ICAM-1, 15.2, BB2, ICAM 1

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.05 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65509-1-PBS targets ICAM-1/CD54 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Rheumatoid synovial cells and human monocytes

• Full Name: intercellular adhesion molecule 1
• Calculated Molecular Weight: 90 kDa
• GenBank Accession Number: BC015969
• Gene Symbol: ICAM-1
• Gene ID (NCBI): 3383
• ENSEMBL Gene ID: ENSG00000090339
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P05362
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C. Stable for one year after shipment.

Background Information

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).