CD62L Recombinant antibody

• 

  WB analysis of Jurkat using 85111-6-RR

  Jurkat cells were subjected to SDS PAGE followed by western blot with 85111-6-RR (SELL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MJ using 85111-6-RR

  MJ cells were subjected to SDS PAGE followed by western blot with 85111-6-RR (SELL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CD62L Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85111-6-RR

Clone No.250646B2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

L-selectin, CD62 antigen-like family member L, gp90 MEL, gp90-MEL, hLHRc

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Tested Applications

• Positive WB detected in: Jurkat cells, MJ cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85111-6-RR targets CD62L in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD62L fusion protein Eg3006
• Full Name: selectin L
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: NM_000655.5
• Gene Symbol: CD62L
• Gene ID (NCBI): 6402
• ENSEMBL Gene ID: ENSG00000188404
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P14151-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD62L, also known as L-selectin or SELL, is a member of the selectin family of adhesion molecules that also include CD62E (E-selectin) and CD62P (P-selectin) (PMID: 2663882, 2473156, 1382078). CD62L is a highly glycosylated protein expressed on the surface of most leukocytes, including lymphocytes, neutrophils, monocytes, eosinophils, hematopoietic progenitor cells, and immature thymocytes (PMID: 1694883, 1688580). It mediates the binding of lymphocytes to high endothelial venules (HEV) of peripheral lymph nodes through interactions with a constitutively expressed ligand, and is also involved in lymphocyte, neutrophil, and monocyte attachment to endothelium at sites of inflammation (PMID: 1382078).