CD63 Monoclonal antibody

CD63 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1


Human and More (2)







Cat no : 67605-1-Ig


CD63, CD63 antigen, CD63 molecule, Granulophysin, ME491, MLA1, OMA81H, Tetraspanin 30, Tspan 30, TSPAN30

Tested Applications

Positive WB detected inHeLa cells, U2OS cells, A549 cells, LNCaP cells, HUVEC cells, THP-1 cells, K-562 cells
Positive IHC detected inhuman tonsillitis tissue, human malignant melanoma tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue, human malignant melanoma tissue, human lymphoma tissue
Positive FC detected inTHP-1 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:10000
Immunohistochemistry (IHC)IHC : 1:350-1:1400
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 25 publications below

Product Information

67605-1-Ig targets CD63 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, goat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD63 fusion protein Ag19690
Full Name CD63 molecule
Calculated molecular weight 26 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC002349
Gene symbol CD63
Gene ID (NCBI) 967
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD63, known as LAMP-3, is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediate signal transduction events that play a role in the regulation of cell development, activation and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it as a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.


Product Specific Protocols
WB protocol for CD63 antibody 67605-1-IgDownload protocol
IHC protocol for CD63 antibody 67605-1-IgDownload protocol
IF protocol for CD63 antibody 67605-1-IgDownload protocol
FC protocol for CD63 antibody 67605-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Stem Cells Int

Exosomes Derived from microRNA-27a-3p Overexpressing Mesenchymal Stem Cells Inhibit the Progression of Liver Cancer through Suppression of Golgi Membrane Protein 1

Authors - Christian Cedric Bongolo

Stem Cell Res Ther

Exosomes derived from human adipose mesenchymal stem cells attenuate hypertrophic scar fibrosis by miR-192-5p/IL-17RA/Smad axis.

Authors - Yan Li

Drug Des Devel Ther

Keloid Patient Plasma-Derived Exosomal hsa_circ_0020792 Promotes Normal Skin Fibroblasts Proliferation, Migration, and Fibrogenesis via Modulating miR-193a-5p and Activating TGF-β1/Smad2/3 Signaling

Authors - Huan Hu

Front Microbiol

Two Small Extracellular Vesicle sRNAs Derived From Mycobacterium tuberculosis Serve as Diagnostic Biomarkers for Active Pulmonary Tuberculosis.

Authors - Geng Lu

Cancer Cell Int

A novel strategy to identify candidate diagnostic and prognostic biomarkers for gastric cancer.

Authors - Lei Liu


Reassessment of the Proteomic Composition and Function of Extracellular Vesicles in the Seminal Plasma.

Authors - Hanshu Wang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Guorong (Verified Customer) (03-22-2022)

Two bands at 30 and 60 kDa was detected

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: ARPE-19
CD63 Antibody Western Blot validation (1:5000 dilution) in ARPE-19 (Cat no:67605-1-Ig)