Validation Data Gallery
|Positive WB detected in||HeLa cells, U2OS cells, MCF-7 cells, A549 cells, K-562 cells, HL-60, THP-1 cells, LNCaP cells, HUVEC cells|
|Positive IHC detected in||human tonsillitis tissue, human malignant melanoma tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human tonsillitis tissue, human malignant melanoma tissue, human lymphoma tissue|
|Positive FC detected in||THP-1 cells|
|Western Blot (WB)||WB : 1:5000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:350-1:1400|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
67605-1-Ig targets CD63 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human samples.
|Cited Reactivity||human, rat, goat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||CD63 fusion protein Ag19690|
|Full Name||CD63 molecule|
|Calculated molecular weight||26 kDa|
|Observed molecular weight||35 kDa|
|GenBank accession number||BC002349|
|Gene ID (NCBI)||967|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CD63 is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediate signal transduction events that play a role in the regulation of cell development, activation and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it as a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.
|Product Specific Protocols|
|WB protocol for CD63 antibody 67605-1-Ig||Download protocol|
|IHC protocol for CD63 antibody 67605-1-Ig||Download protocol|
|IF protocol for CD63 antibody 67605-1-Ig||Download protocol|
|FC protocol for CD63 antibody 67605-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Guorong (Verified Customer) (03-22-2022)
Two bands at 30 and 60 kDa was detected