|Positive WB detected in||HeLa cells, U2OS cells, A549 cells, LNCaP cells, HUVEC cells, THP-1 cells, K-562 cells|
|Positive IHC detected in||human tonsillitis tissue, human malignant melanoma tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human tonsillitis tissue, human malignant melanoma tissue, human lymphoma tissue|
|Western Blot (WB)||WB : 1:5000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:350-1:1400|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
67605-1-Ig targets CD63 in WB, IHC, IF,ELISA applications and shows reactivity with Human samples.
|Host / Isotype||Mouse / IgG1|
|Immunogen||CD63 fusion protein Ag19690|
|Full Name||CD63 molecule|
|Calculated molecular weight||26 kDa|
|Observed molecular weight||35 kDa|
|GenBank accession number||BC002349|
|Gene ID (NCBI)||967|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
CD63, known as LAMP-3, is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediate signal transduction events that play a role in the regulation of cell development, activation and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it as a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.
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