CD63 Monoclonal antibody
CD63 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human and More (2)
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
3D4D1
Cat no : 67605-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, U2OS cells, MCF-7 cells, A549 cells, K-562 cells, HL-60, THP-1 cells, LNCaP cells, HUVEC cells |
| Positive IHC detected in | human tonsillitis tissue, human malignant melanoma tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue, human malignant melanoma tissue, human lymphoma tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:350-1:1400 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 65 publications below |
| IF | See 4 publications below |
| FC | See 3 publications below |
Product Information
67605-1-Ig targets CD63 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat, goat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CD63 fusion protein Ag19690 |
| Full Name | CD63 molecule |
| Calculated Molecular Weight | 26 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC002349 |
| Gene Symbol | CD63 |
| Gene ID (NCBI) | 967 |
| RRID | AB_2882811 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD63 is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediate signal transduction events that play a role in the regulation of cell development, activation and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it as a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD63 antibody 67605-1-Ig | Download protocol |
| IHC protocol for CD63 antibody 67605-1-Ig | Download protocol |
| IF protocol for CD63 antibody 67605-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Lactate dehydrogenase A regulates tumor-macrophage symbiosis to promote glioblastoma progression | ||
Bone Res Interorgan communication in neurogenic heterotopic ossification: the role of brain-derived extracellular vesicles | ||
Drug Des Devel Ther Keloid Patient Plasma-Derived Exosomal hsa_circ_0020792 Promotes Normal Skin Fibroblasts Proliferation, Migration, and Fibrogenesis via Modulating miR-193a-5p and Activating TGF-β1/Smad2/3 Signaling | ||
Oxid Med Cell Longev Adipose-Derived Circulating Exosomes Promote Protection of the Pulmonary Endothelial Barrier by Inhibiting EndMT and Oxidative Stress through Down-Regulation of the TGF-β Pathway: A Potential Explanation for the Obesity Paradox in ARDS. | ||
Aging (Albany NY) MSC-derived exosomes promote recovery from traumatic brain injury via microglia/macrophages in rat. | ||
Aging (Albany NY) Exosomal circRNAs contribute to intestinal development via the VEGF signalling pathway in human term and preterm colostrum. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Marion (Verified Customer) (11-24-2024) | CD63 stains intracellular endosomal vesiclesd in neuronal extensions. We are satisfied with the results.
 |
FH Guorong (Verified Customer) (03-22-2022) | Two bands at 30 and 60 kDa was detected
 |
