CD63 Monoclonal antibody

CD63 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

3D4D1

Cat no : 67605-1-Ig

Synonyms

CD63, CD63 antigen, CD63 molecule, Granulophysin, LAMP 3, ME491, MLA1, OMA81H, Tetraspanin 30, Tspan 30, TSPAN30



Tested Applications

Positive WB detected inHeLa cells, U2OS cells, A549 cells, LNCaP cells, HUVEC cells, THP-1 cells, K-562 cells
Positive IHC detected inhuman tonsillitis tissue, human malignant melanoma tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue, human malignant melanoma tissue, human lymphoma tissue
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:10000
Immunohistochemistry (IHC)IHC : 1:350-1:1400
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 5 publications below

Product Information

67605-1-Ig targets CD63 in WB, IHC, IF,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD63 fusion protein Ag19690
Full Name CD63 molecule
Calculated molecular weight 26 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC002349
Gene symbol CD63
Gene ID (NCBI) 967
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

CD63, known as LAMP-3, is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediate signal transduction events that play a role in the regulation of cell development, activation and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it as a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.

Protocols

Product Specific Protocols
WB protocol for CD63 antibody 67605-1-IgDownload protocol
IHC protocol for CD63 antibody 67605-1-IgDownload protocol
IF protocol for CD63 antibody 67605-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Aging (Albany NY)

MSC-derived exosomes promote recovery from traumatic brain injury via microglia/macrophages in rat.

Authors - Yunfei Chen
humanWB

Exp Cell Res

Exosomes derived from thymic stromal lymphopoietin-treated dendritic cells regulate T helper 17/regulatory T cell differentiation via miR-21/Smad7 axis.

Authors - Qingshan Ji
humanWB

Cancer Cell Int

A novel strategy to identify candidate diagnostic and prognostic biomarkers for gastric cancer.

Authors - Lei Liu
humanWB

Front Microbiol

Two Small Extracellular Vesicle sRNAs Derived From Mycobacterium tuberculosis Serve as Diagnostic Biomarkers for Active Pulmonary Tuberculosis.

Authors - Geng Lu
humanWB

Stem Cell Res Ther

Exosomes derived from human adipose mesenchymal stem cells attenuate hypertrophic scar fibrosis by miR-192-5p/IL-17RA/Smad axis.

Authors - Yan Li