CD63 Monoclonal antibody, PBS Only

CD63 Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3D4D1

Cat no : 67605-1-PBS

Synonyms

CD63, CD63 antigen, CD63 molecule, Granulophysin, LAMP 3, ME491, MLA1, OMA81H, Tetraspanin 30, Tspan 30, TSPAN30

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Validation Data Gallery

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  WB analysis using 67605-1-Ig (same clone as 67605-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67605-1-Ig (CD63 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

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  WB analysis using 67605-1-Ig (same clone as 67605-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67605-1-Ig (CD63 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67605-1-Ig (same clone as 67605-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:700 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67605-1-Ig (same clone as 67605-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:700 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

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  IHC staining of human malignant melanoma using 67605-1-Ig (same clone as 67605-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67605-1-Ig (same clone as 67605-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67605-1-Ig (same clone as 67605-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67605-1-Ig (same clone as 67605-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67605-1-Ig (same clone as 67605-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

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  IHC staining of human malignant melanoma using 67605-1-Ig (same clone as 67605-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 67605-1-Ig (CD63 antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 67605-1-Ig (same clone as 67605-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD63 antibody (67605-1-Ig, Clone: 3D4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 67605-1-Ig (same clone as 67605-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD63 antibody (67605-1-Ig, Clone: 3D4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

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  IF Staining of human lymphoma using 67605-1-Ig (same clone as 67605-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using CD63 antibody (67605-1-Ig, Clone: 3D4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human lymphoma using 67605-1-Ig (same clone as 67605-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human lymphoma tissue using CD63 antibody (67605-1-Ig, Clone: 3D4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 67605-1-Ig (same clone as 67605-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD63 antibody (67605-1-Ig, Clone: 3D4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human malignant melanoma using 67605-1-Ig (same clone as 67605-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human malignant melanoma tissue using CD63 antibody (67605-1-Ig, Clone: 3D4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human malignant melanoma using 67605-1-Ig (same clone as 67605-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human malignant melanoma tissue using CD63 antibody (67605-1-Ig, Clone: 3D4D1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

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  FC experiment of THP-1 using 67605-1-Ig (same clone as 67605-1-PBS)

  1X10^6 THP-1 cells were surface stained with 0.4 ug Anti-Human CD63 (67605-1-Ig, Clone:3D4D1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were not fixed . This data was developed using the same antibody clone with 67605-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67605-1-PBS targets CD63 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD63 fusion protein Ag19690
• Full Name: CD63 molecule
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC002349
• Gene Symbol: CD63
• Gene ID (NCBI): 967
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD63 is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediate signal transduction events that play a role in the regulation of cell development, activation and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it as a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.