Anti-Mouse CD64 Rabbit Recombinant Antibody

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  FC experiment of mouse bone marrow cells using 98218-1-RR

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse CD64 Rabbit Recombinant Antibody (98218-1-RR, Clone: 241809A6) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L), Cells were co-stained with APC Anti-Mouse CD11b (M1/70) (APC-65055, Clone: M1/70). Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using 98218-1-RR

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse CD64 Rabbit Recombinant Antibody (98218-1-RR, Clone: 241809A6) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

CD64 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98218-1-RR

Clone No.241809A6

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

High affinity immunoglobulin gamma Fc receptor I, FcRI, Fcgr1, Fc-gamma RI, Fcg1

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Tested Applications

• Positive FC detected in: mouse bone marrow cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98218-1-RR targets CD64 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: Fc receptor, IgG, high affinity I
• Calculated Molecular Weight: 45 kDa
• GenBank Accession Number: NM_010186.5
• Gene Symbol: Fcgr1
• Gene ID (NCBI): 14129
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P26151
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high-affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses.