CoraLite®647 Anti-Human CD73 (AD2)

CD73 Monoclonal Antibody for FC

Host / Isotype

Mouse / Mouse IgG1 kappa






CoraLite®647 Fluorescent Dye



Cat no : CL647-65162


5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), AD2, CD73, E5NT, Ecto 5 nucleotidase, eN, eNT, NT, NT5, NT5E, NTE

Tested Applications

Positive FC detected inhuman peripheral blood lymphocytes

Recommended dilution

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL647-65162 targets CD73 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / Mouse IgG1 kappa
Class Monoclonal
Type Antibody
Immunogen N/A
Full Name 5'-nucleotidase, ecto (CD73)
Calculated molecular weight 29 kDa, 63 kDa
GenBank accession numberBC015940
Gene symbol NT5E
Gene ID (NCBI) 4907
Conjugate CoraLite®647 Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm / 665 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).


Product Specific Protocols
FC protocol for CL647 CD73 antibody CL647-65162Download protocol
Standard Protocols
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