APC Anti-Human CD73 (AD2)

CD73 Monoclonal Antibody for FC

Host / Isotype

Mouse / Mouse IgG1 kappa

Reactivity

Human

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

AD2

Cat no : APC-65162

Synonyms

5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), CD73, E5NT, Ecto 5 nucleotidase, eN, eNT, NT, NT5, NT5E, NTE



Tested Applications

Positive FC detected inHuman peripheral blood lymphocytes

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

APC-65162 targets CD73 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / Mouse IgG1 kappa
Class Monoclonal
Type Antibody
Immunogen Cells
Full Name 5'-nucleotidase, ecto (CD73)
Calculated molecular weight 29 kDa, 63 kDa
GenBank accession numberBC015940
Gene symbol NT5E
Gene ID (NCBI) 4907
Conjugate APC Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm/660 nm
Form Liquid
Purification Method The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is en ecto-enzyme which catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors is closely involved in cancer progression (PMID: 20179192).

Protocols

Product Specific Protocols
FC protocol for APC CD73 antibody APC-65162Download protocol
Standard Protocols
Click here to view our Standard Protocols