Anti-Human CD73 (AD2)

• 

  FC experiment of human PBMCs using 65162-1-Ig

  1X10^6 human PBMCs were surface co-stained with CL405 Anti-Human CD3 and 0.5 ug Anti-Human CD73 (65162-1-Ig, Clone:AD2) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65162-1-Ig

  1X10^6 human PBMCs were surface stained with 0.5 ug Anti-Human CD73 (65162-1-Ig, Clone:AD2) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red) or Isotype Control. Cells were not fixed. Lymphocytes were gated.

CD73 Monoclonal Antibody for FC

Cat No. 65162-1-Ig

Clone No.AD2

Publications(2)

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Synonyms

NT5E, 5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase, 5' nucleotidase, ecto (CD73)

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 2 publications below

Product Information

65162-1-Ig targets CD73 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Pre-B leukemia cell line

• Full Name: 5'-nucleotidase, ecto (CD73)
• Calculated Molecular Weight: 29 kDa, 63 kDa
• GenBank Accession Number: BC015940
• Gene Symbol: CD73
• Gene ID (NCBI): 4907
• RRID: AB_2881479
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P21589
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	FC	Exp Ther Med; Adipose-derived mesenchymal stem cell exosomes inhibit transforming growth factor-β1-induced collagen synthesis in oral mucosal fibroblasts.; Authors - Junjie Liu
	FC	Dis Markers; Long Noncoding RNA SBF2-AS1 Promotes Abdominal Aortic Aneurysm Formation through the miRNA-520f-3p/SMARCD1 Axis; Authors - Baihe Han