Anti-Human CD73 (AD2)

CD73 Monoclonal Antibody for FC, ELISA

Host / Isotype

Mouse / Mouse IgG1 kappa

Reactivity

Human

Applications

FC, ELISA

Conjugate

Unconjugated

CloneNo.

AD2

Cat no : 65162-1-Ig

Synonyms

5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), CD73, E5NT, Ecto 5 nucleotidase, eN, eNT, NT, NT5, NT5E, NTE



Tested Applications

Positive FC detected inhuman peripheral blood lymphocytes
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

65162-1-Ig targets CD73 in FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / Mouse IgG1 kappa
Class Monoclonal
Type Antibody
Immunogen N/A
Full Name 5'-nucleotidase, ecto (CD73)
Calculated molecular weight 29 kDa, 63 kDa
GenBank accession numberBC015940
Gene symbol NT5E
Gene ID (NCBI) 4907
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C.

Background Information

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is en ecto-enzyme which catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors is closely involved in cancer progression (PMID: 20179192).

Protocols

Product Specific Protocols
FC protocol for CD73 antibody 65162-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols