Tested Applications
| Positive FC detected in | LPS-stimulated rat splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
Biotin-FcA65580 targets CD80 in FC, ELISA applications and shows reactivity with rat samples.
| Tested Reactivity | rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
HTLV-1-transformed Lewis-S1 rat T cell line Predict reactive species |
| Full Name | Cd80 molecule |
| Calculated Molecular Weight | 36 kDa |
| GenBank Accession Number | XM_017597885 |
| Gene Symbol | CD80 |
| Gene ID (NCBI) | 25408 |
| Conjugate | Biotin |
| Excitation/Emission Maxima Wavelengths | - |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | G3V671 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B (PMID: 7545666; 12015893; 16920215).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Biotin CD80 antibody Biotin-FcA65580 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
