Anti-Human CD80 (B7-1) (2D10.4)

CD80 (B7-1) Monoclonal Antibody for FC

Host / Isotype

Mouse / Mouse IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

2D10.4

Cat no : 65083-1-Ig

Synonyms



Tested Applications

Positive FC detected inDaudi cells
Planning an IF experiment? We recommend our CoraLite®647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

65083-1-Ig targets CD80 (B7-1) in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / Mouse IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen N/A
Full Name CD80 molecule
Calculated molecular weight 33 kDa
GenBank accession numberBC042665
Gene symbol CD80
Gene ID (NCBI) 941
Conjugate Unconjugated
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide, pH 7.3.
Storage ConditionsStore at 2-8°C.

Background Information

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)

Protocols

Product Specific Protocols
FC protocol for CD80 (B7-1) antibody 65083-1-IgDownload protocol
Standard Protocols
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