Anti-Human CD86 (BU63) Rabbit IgG Recombinant Antibody, PBS Only

• 

  FC experiment of human PBMCs using 65514-1-RR (same clone as 65514-1-PBS)

  1x10^6 human PBMCs were treated with FC Receptor Block then surface stained with 0.1 ug Anti-Human CD86 Rabbit Recombinant Antibody (65514-1-RR, Clone:BU63) or Rabbit IgG control PolyAb (30000-0-AP), and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65514-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65514-1-PBS

  1x10^6 human PBMCs were treated with FC Receptor Block then surface stained with 0.1 ug Anti-Human CD86 Rabbit Recombinant Antibody (65514-1-RR, Clone:BU63) or Rabbit IgG control PolyAb (30000-0-AP), and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using 65514-1-PBS

  1x10^6 human PBMCs were treated with FC Receptor Block then surface stained with 0.1 ug Anti-Human CD86 Rabbit Recombinant Antibody (65514-1-RR, Clone:BU63) or Rabbit IgG control PolyAb (30000-0-AP), and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using 65514-1-RR (same clone as 65514-1-PBS)

  1x10^6 human PBMCs were treated with FC Receptor Block then surface stained with 0.1 ug Anti-Human CD86 Rabbit Recombinant Antibody (65514-1-RR, Clone:BU63) or Rabbit IgG control PolyAb (30000-0-AP), and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65514-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65514-1-RR (same clone as 65514-1-PBS)

  1x10^6 human PBMCs were treated with FC Receptor Block then surface stained with 0.1 ug Anti-Human CD86 Rabbit Recombinant Antibody (65514-1-RR, Clone:BU63) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) (left and red), or stained with 0.1 ug Anti-Human CD86 Mouse Monoclonal Antibody (Clone:BU63) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65514-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65514-1-PBS

  1x10^6 human PBMCs were treated with FC Receptor Block then surface stained with 0.1 ug Anti-Human CD86 Rabbit Recombinant Antibody (65514-1-RR, Clone:BU63) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7) (left and red), or stained with 0.1 ug Anti-Human CD86 Mouse Monoclonal Antibody (Clone:BU63) and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were not fixed. Monocytes were gated.

CD86 Uni-rAb^® Recombinant Antibody for FC

Cat No. 65514-1-PBS

Clone No.BU63

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

Activation B7 2 antigen, Activation B7-2 antigen, B7 2, B70, BU63

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.1 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65514-1-PBS targets CD86 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  B-lymphoblastoid cell line ARH 77

• Full Name: CD86 molecule
• Calculated Molecular Weight: 329 aa, 38 kDa
• GenBank Accession Number: BC040261
• Gene Symbol: CD86
• Gene ID (NCBI): 942
• ENSEMBL Gene ID: ENSG00000114013
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P42081
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C. Stable for one year after shipment.

Background Information

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)