CD90 / Thy1 Polyclonal antibody

CD90 / Thy1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 27178-1-AP

Synonyms

CD90, CD90 / Thy1, CDw90, Thy 1 antigen, Thy 1 cell surface antigen, Thy 1 membrane glycoprotein, THY1

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Validation Data Gallery

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  WB analysis using 27178-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27178-1-AP (CD90 / Thy1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 27178-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27178-1-AP (CD90 / Thy1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 27178-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27178-1-AP (CD90 / Thy1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 27178-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27178-1-AP (CD90 / Thy1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 27178-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27178-1-AP (CD90 / Thy1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27178-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27178-1-AP (CD90 / Thy1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27178-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27178-1-AP (CD90 / Thy1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 27178-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 27178-1-AP (CD90 / Thy1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 27178-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 27178-1-AP (CD90 / Thy1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: fetal human brain tissue, mouse brain tissue, rat brain tissue, pig brain tissue
• Positive IHC detected in: human colon cancer tissue, human lung cancer tissue, mouse spleen tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 4 publications below

Product Information

27178-1-AP targets CD90 / Thy1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD90 / Thy1 fusion protein Ag25603
• Full Name: Thy-1 cell surface antigen
• Calculated Molecular Weight: 161 aa, 18 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC065559
• Gene Symbol: THY1
• Gene ID (NCBI): 7070
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD90, also known as THY1, is a 25-35 kD protein that is expressed on 1-4% of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. Activation of Thy-1 has been reported to promote T cell activation. It also affects numerous nonimmunologic biological processes, including cellular adhesion, neurite outgrowth, tumor growth, migration, and cell death.

Publications

Species	Application	Title
rat	IF	Neurogastroenterol Motil; Construction and identification of an immortalized rat intestinal smooth muscle cell line.; Authors - Huiling Chen
rat	IF	ACS Nano; Micronano Titanium Accelerates Mesenchymal Stem Cells Aging through the Activation of Senescence-Associated Secretory Phenotype; Authors - Xuan Li
mouse	IF	Mol Immunol; Inhibition of cGAS-STING pathway by stress granules after activation of M2 macrophages by human mesenchymal stem cells against drug induced liver injury; Authors - Yao Wang
human	IF	Stem Cells; Nicotinamide riboside modulates HIF-1 signaling to maintain and enhance odontoblastic differentiation in human dental pulp stem cells; Authors - Peimeng Zhan