Anti-Human CD96 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using 98386-3-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD96 Rabbit RecAb (98386-3-RR, Clone:242964G12) , or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocyte were gated.

• 

  FC experiment of human PBMCs using 98386-3-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD96 Rabbit RecAb (98386-3-RR, Clone:242964G12)(red), or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9)(blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocyte were gated.

CD96 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98386-3-RR

Clone No.242964G12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD96 molecule, Cell surface antigen CD96, T cell surface protein tactile, T cell-activated increased late expression protein, TACTILE

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98386-3-RR targets CD96 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD96 molecule
• Calculated Molecular Weight: 402 aa, 46 kDa
• GenBank Accession Number: NM_198196
• Gene Symbol: CD96
• Gene ID (NCBI): 10225
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P40200
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD96 is a member of the poliovirus receptor (PVR, CD155)-nectin family that includes T cell Ig and ITIM domain (TIGIT) and CD226.. CD96 expression is broadly similar between mice and humans, and is present on a proportion of hematopoietic stem cells, αβ and γδ T-cells, NK cells and a sub-population of B-cells in humans and present on αβ and γδ T-cells, NK cells and NKT cells in mice (PMID: 32043568, 29531070)