Tested Applications
| Positive WB detected in | A431 cells, HeLa cells, HEK-293 cells, COS-7 cells, HepG2 cells, C2C12 cells, C6 cells, Jurkat cells, NIH/3T3 cells, Neuro-2a cells, MCF-7 cells |
| Positive IP detected in | HeLa cells, HEK-293 cells |
| Positive IHC detected in | human cervical cancer tissue, mouse liver tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19532-1-AP targets CDK1/CDC2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
| Tested Reactivity | human, mouse, rat, monkey |
| Cited Reactivity | human, mouse, rat, pig, chicken, bovine, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | cell division cycle 2, G1 to S and G2 to M |
| Calculated Molecular Weight | 34 kDa |
| Observed Molecular Weight | 30-34 kDa |
| GenBank Accession Number | NM_001786 |
| Gene Symbol | CDK1 |
| Gene ID (NCBI) | 983 |
| RRID | AB_10638617 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P06493 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK1 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. It is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Mitotic cyclins stably associate with CDK1 and function as regulatory subunits. CDK1 has 2 isoforms produced by alternative splicing with the molecular mass of 34 kDa and 27 kDa. This antibody may have cross reaction with CDK2.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CDK1/CDC2 antibody 19532-1-AP | Download protocol |
| IF protocol for CDK1/CDC2 antibody 19532-1-AP | Download protocol |
| IHC protocol for CDK1/CDC2 antibody 19532-1-AP | Download protocol |
| IP protocol for CDK1/CDC2 antibody 19532-1-AP | Download protocol |
| WB protocol for CDK1/CDC2 antibody 19532-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CDK1/CDC2 Polyclonal antibody (Cat# 19532-1-AP) has 328 total citations published in journals including Nature Communications, Cell Research, Immunity, Nature Cell Biology, Cell Death & Disease, Oncogene, Advanced Science, and Molecular Therapy. Research fields encompass oncology (breast, lung, liver, gastric, colorectal, and ovarian cancers), cell cycle regulation, autophagy, cardiology, virology, immunology, neuroscience, and pharmacology. The antibody is predominantly used for Western blot, with IHC, IF, and Co-IP also represented.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis
| ||
Mol Cancer Targeting the CD47-HCK-LGALS9 axis disrupts proliferation-immunosuppression coupling in early-stage endometrial cancer. | ||
Cell Res Single-cell RNA-seq highlights intra-tumoral heterogeneity and malignant progression in pancreatic ductal adenocarcinoma. | ||
Immunity Virus-induced deconjugation of ISG15 dysregulates innate immunity and cellular metabolism. | ||
Adv Mater Neutrophil/Leukemia-Tropic Dual-Drug Nanomedicine Potentiates the Treatment of Acute Myeloid Leukemia. | ||
Nat Cell Biol Caspase-2 is a condensate-mediated deubiquitinase in protein quality control |
