CDC34 Polyclonal antibody

CDC34 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 10964-2-AP

Synonyms

CDC34, E2 CDC34, UBC3, UBCH3, UBE2R1, Ubiquitin protein ligase R1

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Validation Data Gallery

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  WB analysis of human pancreas using 10964-2-AP

  human pancreas tissue were subjected to SDS PAGE followed by western blot with 10964-2-AP (CDC34 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10964-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10964-2-AP (CDC34 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 10964-2-AP

  IP result of anti-CDC34 (IP:10964-2-AP, 4ug; Detection:10964-2-AP 1:500) with mouse testis tissue lysate 4000ug.

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  IP experiment of HEK-293 using 10964-2-AP

  IP result of anti-CDC34 (IP:10964-2-AP, 4ug; Detection:10964-2-AP 1:300) with HEK-293 cells lysate 2000 ug.

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  IHC staining of human prostate cancer using 10964-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10964-2-AP (CDC34 antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: human pancreas tissue, human brain tissue
• Positive IP detected in: mouse testis tissue, HEK-293 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

10964-2-AP targets CDC34 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDC34 fusion protein Ag1438
• Full Name: cell division cycle 34 homolog (S. cerevisiae)
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC009850
• Gene Symbol: CDC34
• Gene ID (NCBI): 997
• RRID: AB_2077925
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDC34 is also named as UBCH3, UBE2R1(Ubiquitin-conjugating enzyme E2 R1) and belongs to the ubiquitin-conjugating enzyme family. Cdc34 enzyme is itself a substrate for the type of polyubiquitin modification that is known to cause targeting of proteins for degradation. It is phosphorylated in vivo, providing yet another potential means for control of its activity. And the predominantly nuclear localization of this enzyme suggests that substrate molecules relevant to its cell cycle function are similarly located within the nucleus(PMID:8164658). Human CDC34 could substitute efficiently for yeast CDC34(PMID:8248134). It can form a homodimer(PMID:8383676). This antibody can also recognize CDC34B(UBC3B) which is highly homologous to UBC3 (CDC34) with only 47 amino acid differences.

Publications

Species	Application	Title
human	WB	J Cell Sci; A specific subset of E2 ubiquitin-conjugating enzymes regulate Parkin activation and mitophagy differently.; Authors - Fabienne C Fiesel
mouse	WB	Cell Rep; Cyclin N-Terminal Domain-Containing-1 Coordinates Meiotic Crossover Formation with Cell-Cycle Progression in a Cyclin-Independent Manner.; Authors - Stephen Gray
human	WB	Biochem Biophys Rep; A siRNA screening of UBE2 family demonstrated that UBE2R1 had a high repressive effect on HIV Tat protein; Authors - Muyu Xu ; KD Validated