|Positive WB detected in||human pancreas tissue, human brain tissue|
|Positive IP detected in||mouse testis tissue, HEK-293 cells|
|Positive IHC detected in||human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10964-2-AP targets CDC34 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CDC34 fusion protein Ag1438|
|Full Name||cell division cycle 34 homolog (S. cerevisiae)|
|Calculated molecular weight||34 kDa|
|Observed molecular weight||34 kDa|
|GenBank accession number||BC009850|
|Gene ID (NCBI)||997|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CDC34 is also named as UBCH3, UBE2R1(Ubiquitin-conjugating enzyme E2 R1) and belongs to the ubiquitin-conjugating enzyme family. Cdc34 enzyme is itself a substrate for the type of polyubiquitin modification that is known to cause targeting of proteins for degradation. It is phosphorylated in vivo, providing yet another potential means for control of its activity. And the predominantly nuclear localization of this enzyme suggests that substrate molecules relevant to its cell cycle function are similarly located within the nucleus(PMID:8164658). Human CDC34 could substitute efficiently for yeast CDC34(PMID:8248134). It can form a homodimer(PMID:8383676). This antibody can also recognize CDC34B(UBC3B) which is highly homologous to UBC3 (CDC34) with only 47 amino acid differences.
J Cell Sci
A specific subset of E2 ubiquitin-conjugating enzymes regulate Parkin activation and mitophagy differently.
Cyclin N-Terminal Domain-Containing-1 Coordinates Meiotic Crossover Formation with Cell-Cycle Progression in a Cyclin-Independent Manner.
Biochem Biophys Rep
A siRNA screening of UBE2 family demonstrated that UBE2R1 had a high repressive effect on HIV Tat protein