CDC34 Polyclonal antibody

CDC34 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10964-2-AP

Synonyms

CDC34, E2 CDC34, UBC3, UBCH3, UBE2R1, Ubiquitin protein ligase R1



Tested Applications

Positive WB detected inhuman pancreas tissue, human brain tissue
Positive IP detected inmouse testis tissue, HEK-293 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

10964-2-AP targets CDC34 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDC34 fusion protein Ag1438
Full Name cell division cycle 34 homolog (S. cerevisiae)
Calculated molecular weight 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC009850
Gene symbol CDC34
Gene ID (NCBI) 997
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDC34 is also named as UBCH3, UBE2R1(Ubiquitin-conjugating enzyme E2 R1) and belongs to the ubiquitin-conjugating enzyme family. Cdc34 enzyme is itself a substrate for the type of polyubiquitin modification that is known to cause targeting of proteins for degradation. It is phosphorylated in vivo, providing yet another potential means for control of its activity. And the predominantly nuclear localization of this enzyme suggests that substrate molecules relevant to its cell cycle function are similarly located within the nucleus(PMID:8164658). Human CDC34 could substitute efficiently for yeast CDC34(PMID:8248134). It can form a homodimer(PMID:8383676). This antibody can also recognize CDC34B(UBC3B) which is highly homologous to UBC3 (CDC34) with only 47 amino acid differences.

Protocols

Product Specific Protocols
WB protocol for CDC34 antibody 10964-2-APDownload protocol
IHC protocol for CDC34 antibody 10964-2-APDownload protocol
IP protocol for CDC34 antibody 10964-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Rep

Cyclin N-Terminal Domain-Containing-1 Coordinates Meiotic Crossover Formation with Cell-Cycle Progression in a Cyclin-Independent Manner.

Authors - Stephen Gray
humanWB

J Cell Sci

A specific subset of E2 ubiquitin-conjugating enzymes regulate Parkin activation and mitophagy differently.

Authors - Fabienne C Fiesel