CDC34 Polyclonal antibody, PBS Only

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  WB analysis of human pancreas using 10964-2-AP (same clone as 10964-2-PBS)

  human pancreas tissue were subjected to SDS PAGE followed by western blot with 10964-2-AP (CDC34 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10964-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 10964-2-AP (same clone as 10964-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10964-2-AP (CDC34 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10964-2-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 10964-2-AP (same clone as 10964-2-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 10964-2-AP (CDC34 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10964-2-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 10964-2-AP (same clone as 10964-2-PBS)

  IP result of anti-CDC34 (IP:10964-2-AP, 4ug; Detection:10964-2-AP 1:500) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 10964-2-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 10964-2-AP (same clone as 10964-2-PBS)

  IP result of anti-CDC34 (IP:10964-2-AP, 4ug; Detection:10964-2-AP 1:300) with HEK-293 cells lysate 2000 ug. This data was developed using the same antibody clone with 10964-2-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 10964-2-AP (same clone as 10964-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10964-2-AP (CDC34 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10964-2-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

CDC34 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 10964-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

(E3-independent) E2 ubiquitin-conjugating enzyme R1, E2 CDC34, E2 ubiquitin-conjugating enzyme R1, EC:2.3.2.23, EC:2.3.2.24

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Product Information

10964-2-PBS targets CDC34 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1438

  Product name: Recombinant human CDC34 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-236 aa of BC009850

  Sequence: MARPLVPSSQKALLLELKGLQEEPVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYFKARLKFPIDYPYSPPAFRFLTKMWHPNIYETGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMYRKWKESKGKDREYTDIIRKQVLGTKVDAERDGVKVPTTLAEYCVKTKAPAPDEGSDLFYDDYYEDGEVEEEADSCFGDDEDDSGTEES

• Full Name: cell division cycle 34 homolog (S. cerevisiae)
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC009850
• Gene Symbol: CDC34
• Gene ID (NCBI): 997
• RRID: AB_2077925
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P49427
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CDC34 is also named as UBCH3, UBE2R1(Ubiquitin-conjugating enzyme E2 R1) and belongs to the ubiquitin-conjugating enzyme family. Cdc34 enzyme is itself a substrate for the type of polyubiquitin modification that is known to cause targeting of proteins for degradation. It is phosphorylated in vivo, providing yet another potential means for control of its activity. And the predominantly nuclear localization of this enzyme suggests that substrate molecules relevant to its cell cycle function are similarly located within the nucleus(PMID:8164658). Human CDC34 could substitute efficiently for yeast CDC34(PMID:8248134). It can form a homodimer(PMID:8383676). This antibody can also recognize CDC34B(UBC3B) which is highly homologous to UBC3 (CDC34) with only 47 amino acid differences.