Tested Applications
| Positive WB detected in | C2C12 cell, HeLa cells, C2C12 cells, Jurkat cells |
| Positive IHC detected in | human prostate cancer tissue, human breast cancer tissue, human paracancerous tissue of skin Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10762-1-AP targets CDK1/CDC2 in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig, chicken, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1183 Product name: Recombinant human CDK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-297 aa of BC014563 Sequence: MEDYTKIEKIGEGTYGVVYKGRHKTTGQVVAMKKIRLESEEEGVPSTAIREISLLKELRHPNIVSLQDVLMQDSRLYLIFEFLSMDLKKYLDSIPPGQYMDSSLVKSYLYQILQGIVFCHSRRVLHRDLKPQNLLIDDKGTIKLADFGLARAFGIPIRVYTHEVVTLWYRSPEVLLGSARYSTPVDIWSIGTIFAELATKKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYFNDLDNQIKKM Predict reactive species |
| Full Name | cell division cycle 2, G1 to S and G2 to M |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 30-34 kDa |
| GenBank Accession Number | BC014563 |
| Gene Symbol | CDK1 |
| Gene ID (NCBI) | 983 |
| RRID | AB_2074898 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P06493 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK1 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. It is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Mitotic cyclins stably associate with CDK1 and function as regulatory subunits. CDK1 has 2 isoforms produced by alternative splicing with the molecular mass of 34 kDa and 27 kDa. This antibody may have cross reaction with CDK2.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CDK1/CDC2 antibody 10762-1-AP | Download protocol |
| IF protocol for CDK1/CDC2 antibody 10762-1-AP | Download protocol |
| IHC protocol for CDK1/CDC2 antibody 10762-1-AP | Download protocol |
| WB protocol for CDK1/CDC2 antibody 10762-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CDK1/CDC2 Polyclonal antibody (Cat# 10762-1-AP) has 105 citations. Publications appear in high-impact journals including Nature Communications, Science Advances, Cell Death & Disease, Phytomedicine, Acta Pharmaceutica Sinica B, Advanced Science, Journal of Experimental & Clinical Cancer Research, FASEB Journal, and others. Research fields span oncology (lung, liver, colorectal, breast, gastric, bladder, and head-and-neck cancers), cell cycle regulation, apoptosis, autophagy, ferroptosis, signaling pathways (PI3K/AKT, Wnt/β-catenin, p53), drug discovery, and neuroinflammation.
| Species | Application | Title |
|---|---|---|
Nat Commun Structural mechanisms for centrosomal recruitment and organization of the microtubule nucleator γ-TuRC | ||
Acta Pharm Sin B Design, synthesis, and antitumor activity of novel thioheterocyclic nucleoside derivatives by suppressing the c-MYC pathway | ||
J Exp Clin Cancer Res Nuciferine inhibits the progression of glioblastoma by suppressing the SOX2-AKT/STAT3-Slug signaling pathway. | ||
Adv Sci (Weinh) A HER2-targeted Antibody-Drug Conjugate, RC48-ADC, Exerted Promising Antitumor Efficacy and Safety with Intravesical Instillation in Preclinical Models of Bladder Cancer | ||
Cell Rep Med Tailoring an intravenously injectable oncolytic virus for augmenting radiotherapy |
Reviews
What customers say
The product received a 5-star rating from a user who tested it on mouse tissue using Western Blot. No written comments were provided in the review. Overall, the single review reflects a positive experience with the antibody's performance in WB applications on mouse samples.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Shriya (Verified Customer) (10-26-2025) |
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