CDK1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CDK1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

75

Cat no : 10762-1-AP

Synonyms

CDC2, Cyclin-dependent kinase 1, Cyclin dependent kinase 1, Cell division protein kinase 1, CDKN1

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Validation Data Gallery

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  WB analysis using 10762-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10762-1-AP (CDK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10762-1-AP

  WB result of CDK1 antibody (10762-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK1 transfected HeLa cells.

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  WB analysis of HeLa using 10762-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10762-1-AP (CDK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 10762-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10762-1-AP (CDK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 10762-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10762-1-AP (CDK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10762-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10762-1-AP (CDK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10762-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10762-1-AP (CDK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 10762-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK1 antibody (10762-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  FC experiment of HeLa using 10762-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CDK1 (10762-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: C2C12 cell, HeLa cells, C2C12 cells, Jurkat cells
• Positive IHC detected in: human prostate cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 71 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Product Information

10762-1-AP targets CDK1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, pig, chicken, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDK1 fusion protein Ag1183
• Full Name: cell division cycle 2, G1 to S and G2 to M
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC014563
• Gene Symbol: CDK1
• Gene ID (NCBI): 983
• RRID: AB_2074898
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK1 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. It is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Mitotic cyclins stably associate with CDK1 and function as regulatory subunits. CDK1 has 2 isoforms produced by alternative splicing with the molecular mass of 34 kDa and 27 kDa. This antibody may have cross reaction with CDK2.

Publications

Species	Application	Title
human	WB	J Pharm Anal; MGMT activated by Wnt pathway promotes cisplatin tolerance through inducing slow-cycling cells and nonhomologous end joining in colorectal cancer; Authors - Haowei Zhang
human	WB	Elife; PMID: 30132758; Authors - Tonya Kueck
human	WB	Biomed Pharmacother; Osthole inhibits the PI3K/AKT signaling pathway via activation of PTEN and induces cell cycle arrest and apoptosis in esophageal squamous cell carcinoma.; Authors - Xinbing Zhu
mouse	WB	Mol Ther Nucleic Acids; Inhibition of the lncRNA MIAT prevents podocyte injury and mitotic catastrophe in diabetic nephropathy.; Authors - Ziyang Wang
human	WB	Cell Death Dis; PFKFB3 blockade inhibits hepatocellular carcinoma growth by impairing DNA repair through AKT.; Authors - Wen-Kai Shi
human	WB	Cancers (Basel); Extracellular Vesicle Delivery of TRAIL Eradicates Resistant Tumor Growth in Combination with CDK Inhibition by Dinaciclib.; Authors - Changhong Ke