CDK2 Monoclonal antibody, PBS Only

CDK2 Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4E2E10

Cat no : 60312-1-PBS

Synonyms

CDK2, Cell division protein kinase 2, cyclin dependent kinase 2, p33 protein kinase, p33(CDK2)

---

Validation Data Gallery

• 

  WB analysis of K-562 using 60312-1-Ig (same clone as 60312-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 60312-1-Ig (CDK2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 60312-1-Ig (same clone as 60312-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60312-1-Ig (CDK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 60312-1-Ig (same clone as 60312-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60312-1-Ig (CDK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 60312-1-Ig (same clone as 60312-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60312-1-Ig (CDK2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 60312-1-Ig (same clone as 60312-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60312-1-Ig (CDK2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 60312-1-Ig (same clone as 60312-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CDK2 antibody (60312-1-Ig, Clone: 4E2E10 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 60312-1-Ig (same clone as 60312-1-PBS)

  1X10^6 Jurkat cells were stained with 0.2ug CDK2 antibody (60312-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60312-1-PBS targets CDK2 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen: CDK2 fusion protein Ag21310
• Full Name: cyclin-dependent kinase 2
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC003065
• Gene Symbol: CDK2
• Gene ID (NCBI): 1017
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Caprylic acid/ammonium sulfate precipitation
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment.

Background Information

CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.