CDK5R2/p39 Polyclonal antibody

CDK5R2/p39 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 27058-1-AP

Synonyms

CDK5 activator 2, CDK5R2, CDK5R2/p39, NCK5AI, P39, p39I, p39nck5ai

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Validation Data Gallery

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  WB analysis of Neuro-2a using 27058-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 27058-1-AP (CDK5R2/p39 antibody at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis using 27058-1-AP

  various lysates were subjected to SDS PAGE followed by western blot with 27058-1-AP (CDK5R2/p39 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 27058-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27058-1-AP (CDK5R2/p39 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Neuro-2a cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

27058-1-AP targets CDK5R2/p39 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDK5R2/p39 fusion protein Ag25834
• Full Name: cyclin-dependent kinase 5, regulatory subunit 2 (p39)
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC041771
• Gene Symbol: CDK5R2/p39
• Gene ID (NCBI): 8941
• RRID: AB_2880735
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDK5R2 is a neuron-specific activator of CDK5 kinase. It associates with CDK5 to form an active kinase. This protein and neuron-specific CDK5 activator CDK5R1/p39NCK5A both share limited similarity to cyclins, and thus may define a distinct family of cyclin-dependent kinase activating proteins.

Publications

Species	Application	Title
human	WB,IHC	BMC Cancer; m1A regulator-mediated methylation modification patterns correlated with autophagy to predict the prognosis of hepatocellular carcinoma; Authors - Yingmin Wu