CDK8 Polyclonal antibody

• Featured Product
• KD/KO Validated

CDK8 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 22067-1-AP

Synonyms

Mediator complex subunit CDK8, K35, EC:2.7.11.22, Cyclin-dependent kinase 8, Cyclin dependent kinase 8

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Validation Data Gallery

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  WB analysis using 22067-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22067-1-AP (CDK8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22067-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22067-1-AP (CDK8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22067-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22067-1-AP (CDK8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 22067-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 22067-1-AP (CDK8 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach cancer using 22067-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 22067-1-AP (CDK8 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 22067-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22067-1-AP (CDK8 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 22067-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22067-1-AP (CDK8 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of NIH/3T3 using 22067-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 22067-1-AP (CDK8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of NIH/3T3 using 22067-1-AP

  1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug CDK8 Polyclonal antibody (22067-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, NIH/3T3 cells, mouse embryo tissue
• Positive IHC detected in: human stomach cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: NIH/3T3 cells
• Positive FC (Intra) detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

22067-1-AP targets CDK8 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDK8 fusion protein Ag17148
• Full Name: cyclin-dependent kinase 8
• Calculated Molecular Weight: 464 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC069634
• Gene Symbol: CDK8
• Gene ID (NCBI): 1024
• RRID: AB_2878983
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; Loss of MED12 activates the TGFβ pathway to promote chemoresistance and replication fork stability in BRCA-deficient cells.; Authors - Lindsey M Jackson ; KD Validated
human	WB	PLoS Genet; Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors.; Authors - Emily M Schleicher ; KD Validated
human	WB	Biochim Biophys Acta Gene Regul Mech; PP2A and its adapter protein IER5 induce the DNA-binding ability and target gene expression of E2F1 via dephosphorylation at serine 375; Authors - Hiroto Takeuchi
mouse	WB,IHC	Bull Exp Biol Med; Knockdown of Long Noncoding RNA CCAT2 Suppresses Malignant Phenotype in Human Laryngeal Squamous Cell Carcinoma; Authors - M Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

SITING (Verified Customer) (04-02-2021)

can see band between 50-65kd

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: EMBRYO HEART

CDK8 Antibody Western Blot validation (1:500 dilution) in EMBRYO HEART (Cat no:22067-1-AP)