Validation Data Gallery
|Positive WB detected in||HEK-293 cells, HeLa cells, NIH/3T3 cells, mouse embryo tissue|
|Positive IHC detected in||human stomach cancer tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||NIH/3T3 cells|
|Positive FC detected in||NIH/3T3 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 22067-1-AP is CDK8 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CDK8 fusion protein Ag17148|
|Full Name||cyclin-dependent kinase 8|
|Calculated molecular weight||464 aa, 53 kDa|
|Observed molecular weight||53 kDa|
|GenBank accession number||BC069634|
|Gene ID (NCBI)||1024|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Nucleic Acids Res
Loss of MED12 activates the TGFβ pathway to promote chemoresistance and replication fork stability in BRCA-deficient cells.
Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors.
Biochim Biophys Acta Gene Regul Mech
PP2A and its adapter protein IER5 induce the DNA-binding ability and target gene expression of E2F1 via dephosphorylation at serine 375
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
SITING (Verified Customer) (04-02-2021)
can see band between 50-65kd