CDK8 Polyclonal antibody

CDK8 Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 22067-1-AP

Synonyms

CDK8, Cell division protein kinase 8, cyclin dependent kinase 8, K35, Mediator complex subunit CDK8, Protein kinase K35



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, NIH/3T3 cells, mouse embryo tissue
Positive IHC detected inhuman stomach cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells
Positive FC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22067-1-AP is CDK8 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDK8 fusion protein Ag17148
Full Name cyclin-dependent kinase 8
Calculated molecular weight 464 aa, 53 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC069634
Gene symbol CDK8
Gene ID (NCBI) 1024
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CDK8 antibody 22067-1-APDownload protocol
IHC protocol for CDK8 antibody 22067-1-APDownload protocol
IF protocol for CDK8 antibody 22067-1-APDownload protocol
FC protocol for CDK8 antibody 22067-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS Genet

Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors.

Authors - Emily M Schleicher
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

SITING (Verified Customer) (04-02-2021)

can see band between 50-65kd

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: EMBRYO HEART
CDK8 Antibody Western Blot validation (1:500 dilution) in EMBRYO HEART (Cat no:22067-1-AP)